Purinergic P2Y2 receptors promote hepatocyte resistance to hypoxia

Journal of Hepatology
Rita CariniEmanuele Albano

Abstract

ATP stimulation of purinergic P2 receptors (P2YR and P2XR) regulates several hepatic functions. Here we report the involvement of ATP-mediated signals in enhancing hepatocyte tolerance to lethal stress. The protection given by purinergic agonists was investigated in rat hepatocytes exposed to hypoxia. ATP released after hypotonic stress (200 mOsm/L) as well as P2YR agonists prevented hepatocyte killing by hypoxia with efficiency ranking UTP > ATPgammaS > ADPbetaS, whereas the P2XR agonist, methylene-adenosine-5'-triphosphate, was ineffective. Adenosine-5'-O-3-thiotriphosphate (ATPgammaS; 100 micromol/L) also prevented Na+ -overload in hypoxic cells by inhibiting the Na+/H+ exchanger, without interfering with hypoxic acidosis. ATPgammaS activated Src and promoted a Src-dependent stimulation of both ERK1/2 and p38MAPK. Blocking p38MAPK with SB203580 reverted the protection given by ATPgammaS on both cell viability and Na+ accumulation, whereas ERK1/2 inhibition with PD98058 was ineffective. An increased phosphorylation of ERK1/2 was also evident in untreated hypoxic hepatocytes. PD98058 ameliorated Na+ accumulation and cell death caused by hypoxia. Hepatocyte pre-treatment with ATPgammaS reverted ERK1/2 activation in hypoxic cell...Continue Reading

References

Apr 1, 1997·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·C PeraltaJ Roselló-Catafau
Sep 5, 1997·The Journal of Biological Chemistry·J Orlowski, S Grinstein
Mar 30, 1999·Experimental Cell Research·R CariniE Albano
Dec 22, 2000·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·R CariniE Albano
Mar 10, 2001·The Journal of Biological Chemistry·H ZhangS Pelech
Jun 5, 2002·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Elaine A Irving, Mark Bamford
Jun 27, 2002·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Narci TeohGeoffrey Farrell
Oct 3, 2002·Seminars in Liver Disease·Andrew P Feranchak, J Gregory Fitz
Jan 24, 2003·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Rita CariniEmanuele Albano
Mar 26, 2003·Cardiovascular Research·David G Allen, Xiao-Hui Xiao
Sep 2, 2003·Biochimica Et Biophysica Acta·Erik M Schwiebert, Akos Zsembery
Sep 2, 2003·Gastroenterology·Nazia SelznerPierre-Alain Clavien
Nov 5, 2003·Gastroenterology·Rita Carini, Emanuele Albano
Dec 25, 2003·The Journal of Physiology·R Elaine Bucheimer, Joel Linden
Feb 10, 2004·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Sundararajah ThevanantherSaul J Karpen
Feb 14, 2004·Cardiovascular Research·Stephen C Armstrong
Sep 24, 2004·The International Journal of Biochemistry & Cell Biology·Larry Fliegel
Mar 30, 2005·Biochemical Pharmacology·Smadar YitzhakiAsher Shainberg
Apr 27, 2005·The British Journal of Surgery·N R BangaK R Prasad

❮ Previous
Next ❯

Citations

Apr 23, 2009·Transplantation·Ziv Ben-AriEdith Hochhauser
Dec 25, 2010·World Journal of Gastroenterology : WJG·Elisa AlcheraRita Carini
Nov 26, 2013·Purinergic Signalling·Geoffrey BurnstockSimon C Robson
Jun 29, 2007·FEBS Letters·Emmanuel GonzalesLaurent Combettes
Dec 26, 2006·Journal of Hepatology·Rita CariniEmanuele Albano
May 29, 2008·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Elisa AlcheraRita Carini
May 25, 2007·American Journal of Physiology. Cell Physiology·Shigeo TamiyaNicholas A Delamere
Apr 13, 2019·International Journal of Molecular Sciences·Mónica B Jiménez-CastroCarmen Peralta
Apr 26, 2013·American Journal of Physiology. Cell Physiology·Sergei N OrlovRyszard Grygorczyk
Apr 1, 2020·International Journal of Molecular Sciences·Erandi Velázquez-MirandaFrancisco G Vázquez-Cuevas

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.

Astrocytes & Neurodegeneration

Astrocytes are important for the health and function of the central nervous system. When these cells stop functioning properly, either through gain of function or loss of homeostatic controls, neurodegenerative diseases can occur. Here is the latest research on astrocytes and neurodegeneration.

Anthelmintics

Anthelmintics or antihelminthics are a group of antiparasitic drugs that expel parasitic worms (helminths) and other internal parasites from the body by either stunning or killing them and without causing significant damage to the host. Discover the latest research on anthelmintics here.

AKT Pathway

This feed focuses on the AKT serine/threonine kinase, which is an important signaling pathway involved in processes such as glucose metabolism and cell survival.

African Trypanosomiasis

African trypanosomiasis, also known as sleeping sickness, is an insect-borne parasitic disease of humans and other animals. It is caused by protozoa of the species Trypanosoma brucei and almost invariably progresses to death unless treated. Discover the latest research on African trypanosomiasis here.

Anthelmintics (ASM)

Anthelmintics or antihelminthics are a group of antiparasitic drugs that expel parasitic worms (helminths) and other internal parasites from the body by either stunning or killing them and without causing significant damage to the host. Discover the latest research on anthelmintics here.

Astrocytes

Astrocytes are glial cells that support the blood-brain barrier, facilitate neurotransmission, provide nutrients to neurons, and help repair damaged nervous tissues. Here is the latest research.

© 2022 Meta ULC. All rights reserved