Abstract
Although recent studies have suggested that purinergic receptors are expressed in the anterior pituitary gland, their involvement in the regulation of pituitary hormone gene expression is not completely understood. In the present study, we examined the expression of purinergic receptors and the effects of purinergic receptor ligands on pro-opiomelanocortin (POMC) gene expression, in AtT20 mouse corticotroph cells. We identified the expression of most of the purinergic receptor subtypes (A1, A2, P2X1, 3-7, P2Y1, 2, 4) mRNAs, analysed by the reverse transcriptase-polymerase chain reaction. We also found that adenosine and ATP, two representative and endogenous agonists of A1-3 and P2X/P2Y receptors, respectively, stimulated the 5'-promoter activity of the POMC gene in a dose- and time-related manner. When these ligands were simultaneously used with corticotrophin-releasing hormone (CRH), effects that were more than additive were observed, suggesting an enhancing role of these compounds in CRH-mediated adrenocorticotrophic hormone (ACTH) synthesis. These ligands also stimulated the expression of transcription factors involved in the regulation of the POMC gene, but did not enhance ACTH secretion. Finally, the positive effect of ad...Continue Reading
References
Aug 1, 1985·Neuroendocrinology·M B Anand-SrivastavaM Cantin
Aug 15, 1989·Molecular and Cellular Biochemistry·M B Anand-SrivastavaJ Gutkowska
Oct 1, 1989·Neuroendocrinology·S ScaccianoceE Endröczi
Nov 1, 1982·The Journal of Cell Biology·A Surprenant
Dec 1, 1994·The Journal of Physiology·Y A MeiL Cazin
May 23, 1995·Proceedings of the National Academy of Sciences of the United States of America·Z P ChenS L Lightman
Aug 30, 1996·The Journal of Biological Chemistry·M TomićS S Stojilkovic
Jan 22, 1998·The American Journal of Physiology·C VillalobosJ García-Sancho
Jun 24, 1998·Proceedings of the National Academy of Sciences of the United States of America·W H YuS M McCann
Feb 4, 1999·Regulatory Peptides·M KumariP O Cover
Nov 5, 1999·The Journal of Biological Chemistry·S E HedeI Novak
Mar 29, 2000·Biochemical Pharmacology·M Williams, M F Jarvis
Sep 15, 2000·Brain Research·B J SnellA J Lawrence
Jun 9, 2001·Trends in Endocrinology and Metabolism : TEM·S S Stojilkovic, T Koshimizu
Nov 28, 2001·The European Journal of Neuroscience·M El YacoubiJ M Vaugeois
Mar 9, 2002·Arteriosclerosis, Thrombosis, and Vascular Biology·Geoffrey Burnstock
Feb 13, 2003·Clinical Science·D Aled ReesJack Ham
May 30, 2003·The Journal of Endocrinology·D A ReesJ Ham
Sep 13, 2003·The Journal of Biological Chemistry·Mu-Lan HeStanko S Stojilkovic
Nov 26, 2003·Journal of Neuroendocrinology·S JégouH Vaudry
Dec 25, 2003·The Journal of Physiology·R Elaine Bucheimer, Joel Linden
Feb 24, 2004·Journal of Pharmacological Sciences·Kazuaki Yoshioka, Hiroyasu Nakata
Apr 17, 2004·Kidney International·Helga VitzthumArmin Kurtz
Sep 2, 2004·The Journal of Biological Chemistry·Jun TakasakiMasato Kobori
Citations
Apr 7, 2011·Endocrinology·Shuo LiStanko S Stojilkovic
Nov 23, 2013·Purinergic Signalling·Geoffrey Burnstock
Jun 20, 2006·Pharmacology & Therapeutics·Cinzia VolontéGeoffrey Burnstock
Apr 14, 2016·Neuroscience·Eric PollatzekRüdiger Gerstberger
May 12, 2015·Autonomic Neuroscience : Basic & Clinical·Ivana BjelobabaStanko S Stojilkovic
Jan 1, 2007·Global Public Health·J P Ruger
Apr 13, 2007·Physiological Reviews·Geoffrey Burnstock
Apr 6, 2011·Journal of Endocrinological Investigation·F Pecori GiraldiF Cavagnini
Dec 12, 2019·Purinergic Signalling·Wenqi ZhaoZhenghua Xiang
Jan 15, 2010·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·E C JensenA J Gunn
Jul 8, 2017·Molecular and Cellular Endocrinology·Hana Zemková, Stanko S Stojilkovic
May 22, 2021·Purinergic Signalling·Magdiel SalgadoJuan C Sáez