Purinergic receptor ligands stimulate pro-opiomelanocortin gene expression in AtT-20 pituitary corticotroph cells

Journal of Neuroendocrinology
L-F ZhaoK Hashimoto

Abstract

Although recent studies have suggested that purinergic receptors are expressed in the anterior pituitary gland, their involvement in the regulation of pituitary hormone gene expression is not completely understood. In the present study, we examined the expression of purinergic receptors and the effects of purinergic receptor ligands on pro-opiomelanocortin (POMC) gene expression, in AtT20 mouse corticotroph cells. We identified the expression of most of the purinergic receptor subtypes (A1, A2, P2X1, 3-7, P2Y1, 2, 4) mRNAs, analysed by the reverse transcriptase-polymerase chain reaction. We also found that adenosine and ATP, two representative and endogenous agonists of A1-3 and P2X/P2Y receptors, respectively, stimulated the 5'-promoter activity of the POMC gene in a dose- and time-related manner. When these ligands were simultaneously used with corticotrophin-releasing hormone (CRH), effects that were more than additive were observed, suggesting an enhancing role of these compounds in CRH-mediated adrenocorticotrophic hormone (ACTH) synthesis. These ligands also stimulated the expression of transcription factors involved in the regulation of the POMC gene, but did not enhance ACTH secretion. Finally, the positive effect of ad...Continue Reading

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Citations

Apr 7, 2011·Endocrinology·Shuo LiStanko S Stojilkovic
Nov 23, 2013·Purinergic Signalling·Geoffrey Burnstock
Jun 20, 2006·Pharmacology & Therapeutics·Cinzia VolontéGeoffrey Burnstock
May 12, 2015·Autonomic Neuroscience : Basic & Clinical·Ivana BjelobabaStanko S Stojilkovic
Jan 1, 2007·Global Public Health·J P Ruger
Apr 13, 2007·Physiological Reviews·Geoffrey Burnstock
Apr 6, 2011·Journal of Endocrinological Investigation·F Pecori GiraldiF Cavagnini
Dec 12, 2019·Purinergic Signalling·Wenqi ZhaoZhenghua Xiang
Jan 15, 2010·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·E C JensenA J Gunn
Jul 8, 2017·Molecular and Cellular Endocrinology·Hana Zemková, Stanko S Stojilkovic
May 22, 2021·Purinergic Signalling·Magdiel SalgadoJuan C Sáez

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