Purinergic regulation of guinea pig suburothelial myofibroblasts
Abstract
The Ca(2+)-regulating and electrophysiological properties of guinea-pig suburothelial myofibroblasts have been measured in order to investigate their potential role in the sensation of bladder fullness, due to their strategic position between the urothelium and afferent fibres. Previous work has shown that stretch of the bladder wall releases ATP. Cells that stain positively for vimentin were isolated. About 45% of cells (median membrane capacitance 13.3 pF) exhibited spontaneous depolarizations to about -25 mV with a physiological Cl(-) gradient (frequency 2.6 +/- 1.5 min(-1), duration 14.5 +/- 2.2 s, n= 15). Under voltage-clamp spontaneous inward currents (frequency 1.5 +/- 0.2 min(-1), duration 14.5 +/- 7.0 s, n= 18) were recorded, with a similar reversal potential. The spontaneous currents were preceded by intracellular Ca(2+) transients with a magnitude that was independent of membrane potential. All cells tested responded to ATP by generating an intracellular Ca(2+) transient, followed by inward currents; the currents had a similar reversal potential and slope conductance to their spontaneous counterparts. ATP-generated transients were mimicked by UTP and ADP but not by alpha,beta-methylene-ATP (1-10 microm) or CTP (30 mi...Continue Reading
References
Activation of Ca2+-activated Cl- current by depolarizing steps in rabbit urethral interstitial cells
Citations
Expression of P2X3 purinoceptors in suburothelial myofibroblasts of the normal human urinary bladder
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