Purinergic regulation of guinea pig suburothelial myofibroblasts

The Journal of Physiology
C WuC Fry

Abstract

The Ca(2+)-regulating and electrophysiological properties of guinea-pig suburothelial myofibroblasts have been measured in order to investigate their potential role in the sensation of bladder fullness, due to their strategic position between the urothelium and afferent fibres. Previous work has shown that stretch of the bladder wall releases ATP. Cells that stain positively for vimentin were isolated. About 45% of cells (median membrane capacitance 13.3 pF) exhibited spontaneous depolarizations to about -25 mV with a physiological Cl(-) gradient (frequency 2.6 +/- 1.5 min(-1), duration 14.5 +/- 2.2 s, n= 15). Under voltage-clamp spontaneous inward currents (frequency 1.5 +/- 0.2 min(-1), duration 14.5 +/- 7.0 s, n= 18) were recorded, with a similar reversal potential. The spontaneous currents were preceded by intracellular Ca(2+) transients with a magnitude that was independent of membrane potential. All cells tested responded to ATP by generating an intracellular Ca(2+) transient, followed by inward currents; the currents had a similar reversal potential and slope conductance to their spontaneous counterparts. ATP-generated transients were mimicked by UTP and ADP but not by alpha,beta-methylene-ATP (1-10 microm) or CTP (30 mi...Continue Reading

References

Nov 1, 1970·The Journal of Physiology·S Weidmann
Feb 14, 1998·Progress in Neurobiology·J B FurnessJ C Bornstein
Jul 1, 1998·British Journal of Pharmacology·Y D BogdanovG Burnstock
Dec 1, 1998·The Journal of Physiology·D W Powell
May 8, 2000·The Journal of Urology·H Y LeeG Burnstock
Jul 15, 2000·The Journal of Physiology·G P SergeantN G McHale
Mar 27, 2001·Annual Review of Pharmacology and Toxicology·W C de Groat, N Yoshimura
Apr 5, 2001·American Journal of Physiology. Cell Physiology·C Wu, C H Fry
May 24, 2001·Proceedings of the National Academy of Sciences of the United States of America·M TominagaM Masu
Jul 20, 2002·The Journal of Urology·Karen D McCloskey, Alison M Gurney
Mar 5, 2003·BJU International·O J WisemanD N Landon
Apr 4, 2003·American Journal of Physiology. Cell Physiology·M A HollywoodK D Thornbury

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Citations

Feb 14, 2006·Nature Clinical Practice. Urology·Alison F Brading, Karen D McCloskey
Apr 12, 2006·Nature Clinical Practice. Urology·Michael R Ruggieri
May 21, 2008·Nature Reviews. Neuroscience·Clare J FowlerWilliam C de Groat
Jun 3, 2011·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Thomas J HeppnerMark T Nelson
Jul 18, 2008·American Journal of Physiology. Renal Physiology·Gui-Ping SuiChristopher H Fry
Feb 24, 2012·American Journal of Physiology. Renal Physiology·C H FryA J Kanai
Jan 4, 2008·American Journal of Physiology. Renal Physiology·Louise JohnstonKaren D McCloskey
Apr 19, 2011·BMC Urology·Jacqueline R WoodmanKate H Moore
Feb 22, 2008·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·F Aura KullmannWilliam C de Groat
Dec 19, 2013·Nephro-urology Monthly·Mohammad Sajjad Rahnama'iPhilip E Van Kerrebroeck
Nov 23, 2013·Purinergic Signalling·Geoffrey Burnstock
Jun 28, 2014·BioMed Research International·Y ChengK H Moore
Jul 13, 2013·Neurourology and Urodynamics·Karl-Erik Andersson, Karen D McCloskey
Oct 25, 2011·The Journal of Urology·Michelle LamHikaru Hashitani
Apr 23, 2011·The Journal of Urology·Hikaru HashitaniHikaru Suzuki
Mar 2, 2016·Nature Reviews. Urology·Liana MerrillMargaret A Vizzard
May 22, 2010·The Journal of Urology·Louise JohnstonKaren D McCloskey
May 11, 2010·Experimental Cell Research·Lysianne Follonier CastellaBoris Hinz
Feb 23, 2010·The Journal of Urology·Anthony Kanai, Karl-Erik Andersson
Apr 4, 2009·European Urology·Brian Eyden
Jul 8, 2008·European Urology·Alexander RoosenChristopher H Fry
May 9, 2009·British Journal of Pharmacology·Amarnath TalasilaJohn M Dickenson
Apr 3, 2009·BJU International·Simone GrolJames I Gillespie
May 22, 2009·International Journal of Urology : Official Journal of the Japanese Urological Association·Fei LiuOsamu Yamaguchi
May 9, 2008·International Journal of Urology : Official Journal of the Japanese Urological Association·Isao ArakiMasayuki Takeda
Feb 9, 2006·British Journal of Pharmacology·Clare J Fowler
Jan 26, 2012·Neurourology and Urodynamics·L A BirderC H Fry
Oct 5, 2012·Acta Physiologica·K D McCloskey

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