Purkinje cell dendritic tree development in the absence of excitatory neurotransmission and of brain-derived neurotrophic factor in organotypic slice cultures

Neuroscience
K H AdcockJosef P Kapfhammer

Abstract

The development of the dendritic tree of a neuron is a complex process which is thought to be regulated strongly by signals from afferent fibers. In particular the synaptic activity of afferent fibers and activity-dependent signaling by neurotrophic factors are thought to affect dendritic growth. We have studied Purkinje cell dendritic arbor development in organotypic cultures under suppression of glutamate-mediated excitatory neurotransmission, achieved with multiple combinations of blockers of glutamate receptors. Despite the presence of either single receptor blockers or combinations of blockers predicted to fully suppress glutamate-mediated excitatory neurotransmission Purkinje cell dendritic arbors developed similar to those of control cultures. Furthermore, Purkinje cell dendritic arbors in organotypic cultures from brain-derived neurotrophic factor (BDNF) knockout mice or after pharmacological blockade of trk-receptors also developed in a way similar to control cultures. Our results demonstrate that during the stage of rapid dendritic arbor growth signals from afferent fibers are of minor importance for Purkinje cell dendritic development because a seemingly normal Purkinje cell dendritic tree developed in the absence of...Continue Reading

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Citations

Oct 18, 2007·Brain Cell Biology·Laurens W J BosmanArthur Konnerth
Nov 24, 2011·Molecular Neurobiology·Inge Van HoveLieve Moons
Apr 5, 2005·Brain Research. Developmental Brain Research·Friedrich MetzgerUlrich L M Eisel
Dec 8, 2004·Progress in Histochemistry and Cytochemistry·Josef P Kapfhammer
Dec 15, 2010·PLoS Computational Biology·Christian TetzlaffMarkus Butz
Feb 26, 2005·The Journal of Physiology·Philippe LachampJean-Pierre Kessler
Dec 13, 2006·The European Journal of Neuroscience·Alexandra Sirzen-ZelenskayaJosef P Kapfhammer
Oct 3, 2009·Journal of Neuroscience Research·Olivia S Gugger, Josef P Kapfhammer
Oct 13, 2009·Neurochemical Research·Masahiko Tanaka
Feb 24, 2009·Brain Research·Jill M WeimerDavid A Pearce
Jun 26, 2009·Progress in Neurobiology·Laura LossiAdalberto Merighi

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