Puroindoline-a and alpha1-purothionin form ion channels in giant liposomes but exert different toxic actions on murine cells

The FEBS Journal
Paola LlanosE Benoit

Abstract

Puroindoline-a (PIN-a) and alpha1-purothionin (alpha1-PTH), isolated from wheat endosperm of Triticum aestivum sp., have been suggested to play a role in plant defence mechanisms against phytopathogenic organisms. We investigated their ability to form pores when incorporated into giant liposomes using the patch-clamp technique. PIN-a formed cationic channels (approximately 15 pS) with the following selectivity K(+) > Na(+) > Cl(-). Also, alpha1-PTH formed channels of approximately 46 pS and 125 pS at +100 mV, the selectivity of which was Ca(2+) > Na(+) approximately K(+) > Cl(-) and Cl(-) > Na(+), respectively. In isolated mouse neuromuscular preparations, alpha1-PTH induced muscle membrane depolarization, leading to blockade of synaptic transmission and directly elicited muscle twitches. Also, alpha1-PTH caused swelling of differentiated neuroblastoma NG108-15 cells, membrane bleb formation, and disorganization of F-actin. In contrast, similar concentrations of PIN-a had no detectable effects. The cytotoxic actions of alpha1-PTH on mammalian cells may be explained by its ability to induce cationic-selective channels.

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Citations

Jun 19, 2013·Journal of Agricultural and Food Chemistry·Michael R SandersRebecca J Green
Aug 27, 2011·Physical Chemistry Chemical Physics : PCCP·Luke A CliftonRebecca J Green
Apr 9, 2013·Biochimica Et Biophysica Acta·Evan F HaneyHans J Vogel
Apr 28, 2017·Langmuir : the ACS Journal of Surfaces and Colloids·Michael R SandersRebecca J Green
May 28, 2019·World Journal of Microbiology & Biotechnology·Craig F Morris
Apr 29, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·Florent SandrasChristine Grauby-Heywang

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