Puromycin-sensitive aminopeptidase limits MHC class I presentation in dendritic cells but does not affect CD8 T cell responses during viral infections

The Journal of Immunology : Official Journal of the American Association of Immunologists
Charles F TowneKenneth L Rock

Abstract

Previous experiments using enzyme inhibitors, cell lysates, and purified enzyme have suggested that puromycin-sensitive aminopeptidase (PSA) plays a role in creating and destroying MHC class I-presented peptides although its precise contribution to these processes is unknown. To examine the importance of this enzyme in MHC class I Ag presentation, we have generated PSA-deficient mice and cell lines from these animals. PSA-deficient mice are smaller and do not reproduce as well as wild type mice. In addition, dendritic cells from PSA-deficient mice display more MHC class I molecules on the cell surface, suggesting that PSA normally limits Ag presentation by destroying certain peptides in these key APCs. Surprisingly, MHC class I levels are not altered on other PSA-deficient cells and the processing and presentation of peptide precursors in PSA-deficient fibroblasts is normal. Moreover, PSA-deficient mice have normal numbers of T cells in the periphery, and respond as well as wild type mice to eight epitopes from three viruses. These data indicate that PSA may play a role in limiting MHC class I Ag presentation in dendritic cells in vivo but that it is not essential for generating most MHC class I-presented peptides or for stimul...Continue Reading

References

Jan 1, 1981·Journal of Neurochemistry·L B Hersh, J F McKelvy
Nov 10, 1995·The Journal of Biological Chemistry·D B ConstamA Fontana
Aug 1, 1996·European Journal of Immunology·J O KoopmannF Momburg
Oct 6, 1997·Proceedings of the National Academy of Sciences of the United States of America·A CraiuK L Rock
May 18, 1999·Biochemical and Biophysical Research Communications·M W ThompsonL B Hersh
Mar 23, 2001·Nature Immunology·L StoltzeH Schild
Feb 23, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Andrew T Miller, Leslie J Berg
Oct 9, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Frédéric LévyCatherine Servis
Feb 25, 2003·Nature Immunology·Ulrike SeifertAnne Hosmalin
May 6, 2003·Nature Biotechnology·David M ValenzuelaGeorge D Yancopoulos
Mar 23, 2004·Annual Review of Immunology·Raymond M WelshEva Szomolanyi-Tsuda
Jun 30, 2004·Nature Immunology·Kenneth L RockAlfred L Goldberg
Dec 30, 2004·The Journal of Experimental Medicine·David C TscharkeJonathan W Yewdell
Nov 8, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Charles F TowneKenneth L Rock
Dec 24, 2005·Science·Ramunas M Vabulas, F Ulrich Hartl
Jun 7, 2006·Proceedings of the National Academy of Sciences of the United States of America·Ian A YorkKenneth L Rock
Jul 20, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Ian A YorkKenneth L Rock

❮ Previous
Next ❯

Citations

Apr 30, 2015·Bioscience, Biotechnology, and Biochemistry·Dong-Gi LeeJong-Soon Choi
Oct 21, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Masahiro KawaharaKenneth L Rock
Mar 31, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Arron HearnKenneth L Rock
Aug 16, 2013·Journal of Biochemistry·Akira Hattori, Masafumi Tsujimoto
Sep 18, 2010·Journal of Oncology·Giulio PretaRickard Glas
Jan 31, 2009·Antioxidants & Redox Signaling·Youngkyun KimKwangseog Ahn
Dec 24, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Kenneth L RockLianjun Shen
Mar 11, 2011·Cellular and Molecular Life Sciences : CMLS·Peter van Endert
Nov 18, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Eunkyung KimKwangseog Ahn
Nov 20, 2013·Proceedings of the National Academy of Sciences of the United States of America·Efthalia ZervoudiEfstratios Stratikos
May 16, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Shao Chong ZhangSylvie Le Gall
Sep 15, 2012·Medical Microbiology and Immunology·Nadja AkkadHansjörg Schild
Jan 5, 2014·Amino Acids·Sarina M HitzerdGodefridus J Peters
Sep 11, 2010·Human Molecular Genetics·Fiona M MenziesDavid C Rubinsztein
Jun 19, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Sabrina UrbanUlrike Seifert
Mar 10, 2011·Cellular and Molecular Life Sciences : CMLS·Nathalie Vigneron, Benoît J Van den Eynde
Mar 26, 2014·Development Genes and Evolution·Mark J AlthoffChris Trzepacz
Feb 19, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Ralf M LeonhardtMichael R Knittler
Dec 9, 2009·Antiviral Research·Ulrika TehlerGordon L Amidon

❮ Previous
Next ❯

Related Concepts

Related Papers

The Journal of Immunology : Official Journal of the American Association of Immunologists
Masahiro KawaharaKenneth L Rock
The Journal of Immunology : Official Journal of the American Association of Immunologists
Charles F TowneKenneth L Rock
The Journal of Immunology : Official Journal of the American Association of Immunologists
Charles F TowneKenneth L Rock
The Journal of Immunology : Official Journal of the American Association of Immunologists
Arron HearnKenneth L Rock
© 2021 Meta ULC. All rights reserved