Purple-grained barley (Hordeum vulgare L.): marker-assisted development of NILs for investigating peculiarities of the anthocyanin biosynthesis regulatory network

BMC Plant Biology
Elena I GordeevaOlesya Yu Shoeva

Abstract

Anthocyanins are plants secondary metabolites important for plant adaptation to severe environments and potentially beneficial to human health. Purple colour of barley grain is caused by the pigments synthesized in pericarp. One or two genes determine the trait. One of them is Ant2 mapped on chromosome 2HL and is known to encode transcription factor (TF) with a bHLH domain. In plants, bHLH regulates anthocyanin biosynthesis together with TF harboring an R2R3-MYB domain. In wheat, the R2R3-MYBs responsible for purple colour of grain pericarp are encoded by the homoallelic series of the Pp-1 genes that were mapped on the short arms of chromosomes 7. In barley, in orthologous positions to wheat's Pp-1, the Ant1 gene determining red colour of leaf sheath has been mapped. In the current study, we tested whether Ant1 has pleiotropic effect not only on leaf sheath colour but also on pericarp pigmentation. А set of near isogenic lines (NILs) carrying different combinations of alleles at the Ant1 and Ant2 loci was created using markers-assisted backcrossing approach. The dominant alleles of both the Ant1 and Ant2 genes are required for anthocyanin accumulation in pericarp. A qRT-PCR analysis of the Ant genes in lemma and pericarp of the...Continue Reading

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Citations

Apr 23, 2020·Oxidative Medicine and Cellular Longevity·Yawen ZengJiazhen Yang
Mar 1, 2019·BMC Plant Biology·Yuriy L OrlovAlex V Kochetov
Jan 7, 2021·Plants·Igor G Loskutov, Elena K Khlestkina
Apr 29, 2021·Journal of Agricultural and Food Chemistry·Chenlu ZhouNing Han
Oct 16, 2020·Journal of Agricultural and Food Chemistry·Boyan TangGuoping Chen
Sep 3, 2021·Biotechnology & Genetic Engineering Reviews·Bharati LapWricha Tyagi

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Methods Mentioned

BETA
PCR
dissection
PCRs

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