Pushing, pulling, and squeezing our way to understanding mechanotransduction

Methods : a Companion to Methods in Enzymology
Michael J SiedlikCeleste M Nelson

Abstract

Mechanotransduction is often described in the context of force-induced changes in molecular conformation, but molecular-scale mechanical stimuli arise in vivo in the context of complex, multicellular tissue structures. For this reason, we highlight and review experimental methods for investigating mechanotransduction across multiple length scales. We begin by discussing techniques that probe the response of individual molecules to applied force. We then move up in length scale to highlight techniques aimed at uncovering how cells transduce mechanical stimuli into biochemical activity. Finally, we discuss approaches for determining how these stimuli arise in multicellular structures. We expect that future work will combine techniques across these length scales to provide a more comprehensive understanding of mechanotransduction.

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Citations

Feb 21, 2016·Methods : a Companion to Methods in Enzymology·Nicolas BorghiChristophe Lavelle
Jul 26, 2016·Biomedical Optics Express·Mohammad SarsharBahman Anvari
Dec 23, 2016·Cellular and Molecular Life Sciences : CMLS·Bryan A NergerCeleste M Nelson
Feb 23, 2018·The Journal of Cell Biology·Florence Broders-BondonEmmanuel Farge
Apr 20, 2019·PLoS Neglected Tropical Diseases·Perran A RossAry A Hoffmann
Sep 25, 2019·Nature Methods·Robert PrevedelGiuseppe Antonacci
Apr 10, 2020·Advanced Healthcare Materials·Cheavar A Blair, Beth L Pruitt
Oct 17, 2019·Biophysics Reviews·Matteo ChighizolaCarsten Schulte
Mar 30, 2017·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Guy B Blanchard
Jul 5, 2017·Proceedings of the National Academy of Sciences of the United States of America·Kevin C HartMartijn Gloerich
Nov 1, 2020·Biomaterials·Berna ÖzkaleDavid J Mooney
Apr 4, 2021·Cells·Ashwini ShindeTuhin Subhra Santra

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