Pushing the boundaries of optoacoustic microscopy by total impulse response characterization.

Nature Communications
Markus SeegerVasilis Ntziachristos

Abstract

Optical microscopy improves in resolution and signal-to-noise ratio by correcting for the system's point spread function; a measure of how a point source is resolved, typically determined by imaging nanospheres. Optical-resolution optoacoustic (photoacoustic) microscopy could be similarly corrected, especially to account for the spatially-dependent signal distortions induced by the acoustic detection and the time-resolved and bi-polar nature of optoacoustic signals. Correction algorithms must therefore include the spatial dependence of signals' origins and profiles in time, i.e. the four-dimensional total impulse response (TIR). However, such corrections have been so far impeded by a lack of efficient TIR-characterization methods. We introduce high-quality TIR determination based on spatially-distributed optoacoustic point sources (SOAPs), produced by scanning an optical focus on an axially-translatable 250 nm gold layer. Using a spatially-dependent TIR-correction improves the signal-to-noise ratio by >10 dB and the axial resolution by ~30%. This accomplishment displays a new performance paradigm for optoacoustic microscopy.

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Citations

Oct 1, 2020·Visual Computing for Industry, Biomedicine and Art·Yuanzheng MaSihua Yang
Jan 28, 2021·Scientific Reports·Markus SeegerVasilis Ntziachristos
Feb 3, 2021·Nature Materials·Arash FarhadiMikhail G Shapiro
Jul 6, 2021·Frontiers in Neuroscience·Silviu-Vasile Bodea, Gil Gregor Westmeyer
Jul 11, 2021·The Review of Scientific Instruments·K HassF E Veiras

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Methods Mentioned

BETA
infrared microscopy

Software Mentioned

Matlab

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