Putative "acylaminoacyl" peptidases from Streptomyces griseus and S. coelicolor display "aminopeptidase" activities with distinct substrate specificities and sensitivities to reducing reagent

Biochimica Et Biophysica Acta
Hirokazu UsukiTadashi Hatanaka

Abstract

Aminopeptidases from Streptomyces griseus (SGRAP) and S. coelicolor (SCOAP) were cloned and characterized to clarify their biochemical characteristics. Although both enzymes had been annotated as putative oligopeptidases of family S9 enzymes, they showed "aminopeptidase" activities, not "oligopeptidase" activities. Although their deduced amino acid sequences showed high similarity (69% overall sequence homology), they showed distinct substrate specificities and sensitivities to the reducing reagent dithiothreitol (DTT). The reaction pH and addition of DTT dramatically affected the substrate preference of SGRAP. Furthermore, SCOAP selectively hydrolyzed phenyalanine p-nitroanilide (Phe-pNA) in the presence or absence of DTT. The chimera protein between SGRAP and SCOAP was constructed to identify the region responsible for the properties described above. Furthermore, Cys(409) of SCOAP was identified as a functional residue responsible for activation by reducing reagent DTT.

References

Sep 3, 1999·The Journal of Biological Chemistry·R E MortyB A Burleigh
Mar 28, 2002·Cellular and Molecular Life Sciences : CMLS·L Polgár
Feb 7, 2003·Applied and Environmental Microbiology·Daniëlle E J W BastenPeter J Schaap
Jul 3, 2003·Nature Reviews. Genetics·Xose S PuenteCarlos López-Otín
Feb 5, 2005·Applied and Environmental Microbiology·Koichi MoriTadashi Hatanaka
May 19, 2005·The Journal of Biological Chemistry·Yoshiko UesugiTadashi Hatanaka
May 9, 2006·Cell Biochemistry and Biophysics·Dean Rea, Vilmos Fülöp
Oct 13, 2006·Clinica Chimica Acta; International Journal of Clinical Chemistry·Inger BrandtAnne-Marie Lambeir

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Citations

May 26, 2010·Applied Microbiology and Biotechnology·Jiro ArimaTadanori Aimi
Apr 27, 2010·Applied and Environmental Microbiology·Jiro ArimaTadashi Hatanaka
Sep 29, 2011·Applied and Environmental Microbiology·Jiro ArimaNobuhiro Mori

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