Putative identification of an amphipathic alpha-helical sequence in hemolysin of Escherichia coli (HlyA) involved in transmembrane pore formation

Biological Chemistry
Angela ValevaIwan Walev

Abstract

Abstract Escherichia coli hemolysin is a pore-forming protein belonging to the RTX toxin family. Cysteine scanning mutagenesis was performed to characterize the putative pore-forming domain of the molecule. A single cysteine residue was introduced at 48 positions within the sequence spanning residues 170-400 and labeled with the polarity-sensitive dye badan. Spectrofluorimetric analyses indicated that several amino acids in this domain are inserted into the lipid bilayer during pore formation. An amphipathic alpha-helix spanning residues 272-298 was identified that may line the aqueous pore. The importance of this sequence was highlighted by the introduction of two prolines at positions 284 and 287. Disruption of the helix structure did not affect binding properties, but totally abolished the hemolytic activity of the molecule.

References

Apr 1, 1991·Molecular & General Genetics : MGG·A LudwigW Goebel
Jul 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·T Felmlee, R A Welch
Jun 1, 1988·European Journal of Epidemiology·S BhakdiI B Holland
Feb 1, 1987·Molecular & General Genetics : MGG·A LudwigW Goebel
Oct 1, 1993·Molecular & General Genetics : MGG·A LudwigW Goebel
Oct 25, 1996·The Journal of Biological Chemistry·M PalmerS Bhakdi
Jan 30, 1997·Nature·M WienerR M Stroud
Feb 27, 1997·Nature·C PetosaR C Liddington
Aug 6, 2000·The Biochemical Journal·B Promdonkoy, D J Ellar
Jan 30, 2002·Biochemistry·Shilla NassiAlan Finkelstein
Apr 10, 2002·The Journal of Biological Chemistry·Aitziber L CortajarenaHelena Ostolaza
Jun 26, 2003·Nucleic Acids Research·Burkhard Rost, Jinfeng Liu
Sep 1, 2005·The Journal of Biological Chemistry·Angela ValevaSucharit Bhakdi
Dec 27, 2005·The Journal of Biological Chemistry·Lissete Sánchez-MagranerHelena Ostolaza
Mar 28, 2006·Current Opinion in Structural Biology·Sarah J Tilley, Helen R Saibil
Mar 10, 2007·The Journal of Biological Chemistry·Marek BaslerRadim Osicka

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.