Putative roles as oncogene or tumour suppressor of the Mid-clustered microRNAs in Gallid alphaherpesvirus 2 (GaHV2) induced Marek's disease lymphomagenesis

The Journal of General Virology
Man TengJun Luo

Abstract

In the last decade, numerous microRNAs (miRNAs) have been identified in diverse virus families, particularly in herpesviruses. Gallid alphaherpesvirus 2 (GaHV2) is a representative oncogenic alphaherpesvirus that induces rapid-onset T-cell lymphomas in its natural hosts, namely Marek's disease (MD). In the GaHV2 genome there are 26 mature miRNAs derived from 14 precursors assembled into three clusters, namely the Meq-cluster, Mid-cluster and LAT-cluster. Several GaHV2 miRNAs, especially those in the Meq-cluster (e.g. miR-M4-5p), have been demonstrated to be critical in MD pathogenesis and/or tumorigenesis. Interestingly the downstream Mid-cluster is regulated and transcribed by the same promoter as the Meq-cluster in the latent phase of the infection, but the role of these Mid-clustered miRNAs in GaHV2 biology remains unclear. We have generated the deletion mutants of the Mid-cluster and of its associated individual miRNAs in GX0101 virus, a very virulent GaHV2 strain, and demonstrated that the Mid-clustered miRNAs are not essential for virus replication. Using GaHV2-infected chickens as an animal model, we found that, compared with parental GX0101 virus, the individual deletion of miR-M31 decreased the mortality and gross tumo...Continue Reading

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Citations

Jan 4, 2019·Journal of Cellular Physiology·Homa MollaeiZeinab Rostami
Nov 7, 2019·International Journal of Molecular Sciences·Linyi Zhou, Shijun J Zheng
Jan 24, 2021·Research in Veterinary Science·Zhaobo ZhangLiping Han
Oct 30, 2020·Cancers·Magdalena GebertRafał Bartoszewski

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Datasets Mentioned

BETA
GX0101

Methods Mentioned

BETA
reverse-transcription PCR
PCR
electrophoresis
transfection

Software Mentioned

GraphPad
GraphPad Prism
RNAhybrid

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