Putative storage root specific promoters from cassava and yam: cloning and evaluation in transgenic carrots as a model system

Plant Cell Reports
Jacobo ArangoSalim Al-Babili

Abstract

A prerequisite for biotechnological improvements of storage roots is the availability of tissue-specific promoters enabling high expression of transgenes. In this work, we cloned two genomic fragments, pMe1 and pDJ3S, controlling the expression of a gene with unknown function from cassava (Manihot esculenta) and of the storage protein dioscorin 3 small subunit gene from yam (Dioscorea japonica), respectively. Using beta-glucuronidase as a reporter, the activities of pMe1 and pDJ3S were evaluated in independent transgenic carrot lines and compared to the constitutive CaMV35S and the previously described cassava p15 promoters. Activities of pMe1 and pDJ3S in storage roots were assessed using quantitative GUS assays that showed pDJ3S as the most active one. To determine organ specificities, uidA transcript levels in leaves, stems and roots were measured by real-time RT-PCR analyses showing highest storage root specificity for pDJ3S. Root cross sections revealed that pMe1 was highly active in secondary xylem. In contrast, pDJ3S was active in all root tissues except for the central xylem. The expression patterns caused by the cassava p15 promoter in carrot storage roots were consistent with its previously described activities for th...Continue Reading

References

Dec 10, 1998·Nucleic Acids Research·K HigoT Korenaga
Dec 22, 1999·The Plant Journal : for Cell and Molecular Biology·C K ShewmakerD Y Ke
Feb 24, 2001·Transgenic Research·P ZhangJ Puonti-Kaerlas
May 6, 2003·Annals of Botany·Peter R Shewry
Mar 9, 2004·Analytical Biochemistry·Huijun Tian, Howard J Edenberg
Mar 30, 2004·The Journal of Biological Chemistry·Mariam GaidamashviliKoji Muramoto
May 27, 2004·Journal of Agricultural and Food Chemistry·Rebecca L SurlesSherry A Tanumihardjo
Jan 5, 2005·Plant Molecular Biology·Hernán CeballosAlfred G O Dixon
Jan 5, 2005·Plant Molecular Biology·Camilo LopezValérie Verdier
Mar 29, 2005·Nature Biotechnology·Jacqueline A PaineRachel Drake
Dec 21, 2006·Plant Biotechnology Journal·Uzoma IhemereRichard Sayre
Nov 6, 2008·Nucleic Acids Research·Aron Marchler-BauerStephen H Bryant
Jul 1, 2009·Comprehensive Reviews in Food Science and Food Safety·Julie A MontagnacSherry A Tanumihardjo

❮ Previous
Next ❯

Citations

Nov 13, 2012·Current Opinion in Biotechnology·Jan JirschitzkaJohn Charles D'Auria
Dec 23, 2015·Genome Génome / Conseil National De Recherches Canada·Julia Lambret-FrottéMarcio Alves-Ferreira
May 14, 2011·Journal of Integrative Plant Biology·Jia LiuPeng Zhang
Jan 13, 2015·Molecular Biotechnology·Sergio Rosales-Mendoza, Marlene Anahí Tello-Olea
Mar 20, 2013·International Journal of Molecular Sciences·Zhong ChenXin-Min An
Apr 4, 2021·Plants·Inger Bæksted HolmeHenrik Brinch-Pedersen

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.