Putative use of a Bacillus subtilis L194 strain for biocontrol of Phoma medicaginis in Medicago truncatula seedlings

Research in Microbiology
Imen Ben SlimeneFerid Limam

Abstract

An antagonist L194 strain against Phoma medicaginis pathogenic fungi was isolated from Tunisian soil (vicinity of Tunis) and identified as Bacillus subtilis based on biochemical characteristics and partial 16S rDNA sequence. When cells were grown in a minimal medium for 24 h, spore culture supernatant exhibited 2-fold higher antifungal activity than vegetative cells. MALDI-TOF mass spectrometry analysis showed that L194 spores produced mainly iturins, surfactins and fengycins with long-chain fatty acids, and other not yet identified compounds. Both vegetative cells and spores of L194 efficiently reduced germination of P. medicaginis conidia. As revealed by atomic force microscopy, L194 spores modified conidia morphology from a regular to a deflated shape. Data suggest that lipopeptides interacted with the cytoplasmic membrane, causing pore formation. In vivo, L194 spores were highly protective against P. medicaginis by reducing disease symptoms and alleviating growth disturbance of Medicago truncatula seedlings. As a whole, the lipopeptide-producing L194 strain may be successfully used in biocontrol of plant diseases induced by phytopathogenic fungi such as P. medicaginis.

References

May 10, 1974·Annals of the New York Academy of Sciences·A L Demain
Jan 1, 1970·Annual Review of Microbiology·R S HansonA A Yousten
Mar 1, 1993·Molecular Microbiology·M A MarahielP Zuber
Aug 1, 1994·Mycopathologia·M A KlichJ M Bland
Aug 25, 1999·Current Opinion in Plant Biology·D R Cook
Apr 28, 2001·Journal of Experimental Botany·J M Whipps
Oct 10, 2001·Journal of Bacteriology·K TsugeM Shoda
Oct 14, 2004·Journal of Applied Microbiology·P I KimY-T Chi
Jun 14, 2005·Biochimica Et Biophysica Acta·Francisco J ArandaAntonio Ortiz
Jul 26, 2008·Archives of Microbiology·Augusto EtchegarayOmar Teschke
Dec 4, 2008·Mycological Research·László IrinyiErzsébet Sándor

❮ Previous
Next ❯

Citations

Oct 31, 2018·Environmental Science and Pollution Research International·Dorra Ben AbdallahOlfa Frikha-Gargouri

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacillus Cereus Infection

Bacillus Cereus is a gram-positive bacteria that is the cause of some foodborne illnesses and leads to diarrhea and vomiting. Discover the latest research on Bacillus Cereus Infection here.

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.