PMID: 37156Apr 1, 1979

Putrescine-oxidase activity in adult bovine serum and fetal bovine serum

In Vitro
W A Gahl, H C Pitot

Abstract

Putrescine-oxidase activity was found in fetal bovine serum (FBS) with a pH optimum of 8.0 and in adult bovine serum (ABS) with a pH optimum of 9.8. The crude FBS enzyme had a KM for putrescine of 2.58 x 10(-6) M and a Vmax of 0.53 nmol per hr per 50 microliter serum. Aminoguanidine competitively inhibited the enzyme with a KI of 1.8 x 10(-8) M. Spermidine and spermine proved competitive inhibitors of putrescine for both the FBS and the crude ABS putrescine oxidases. The Vmax for the ABS putrescine oxidase was 2.10 nmol per hr per 50 microliter serum, and the KM for putrescine, 50.3 x 10(-6) M. The K1 of the ABS putrescine oxidase for aminoguanidine was 41 x 10(-6) M. On the basis of both the KM and KI values, the adult serum enzyme, at its optimal pH of 9.8, bound spermidine and spermine more avidly than the smaller putrescine and aminoguanidine; whereas the FBS enzyme, at pH 8.0, bound aminoguanidine and putrescine more tightly than the larger polyamines. Each of the enzymes retained over 80% of its activity after heating at 56 degrees C for 30 min. Applications of these data to the study of polyamines in tissue culture and to the purification of diamine oxidases are discussed.

References

Jan 15, 1975·Biochemical Pharmacology·C M Hill, W G Bardsley
Aug 26, 1975·Biochimica Et Biophysica Acta·S B Baylin, S Margolis
Jun 1, 1977·The Journal of Pediatrics·D W LungrenJ W Willison
Oct 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·R H FillingameD R Morris
Jun 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·J S HellerE S Canellakis
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Jan 28, 1959·The Journal of Physiology·H BLASCHKO, R HAWES
Sep 1, 1961·Archives of Biochemistry and Biophysics·R A ALARCONE J MODEST
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Citations

Jan 1, 1991·Journal of Cellular Biochemistry·C C Wang
Apr 1, 1987·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·E Niskanen, W W Wharton

Related Concepts

Oxidoreductases Acting on CH-NH Group Donors
Metazoa
Bos indicus
Umbilical Cord Blood
Guanidines
Hydrogen-Ion Concentration
Putrescine
Spermidine
Spermine

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