PV1 Protein from Plasmodium falciparum Exhibits Chaperone-Like Functions and Cooperates with Hsp100s.

International Journal of Molecular Sciences
Kazuaki HakamadaMasafumi Yohda

Abstract

Plasmodium falciparum parasitophorous vacuolar protein 1 (PfPV1), a protein unique to malaria parasites, is localized in the parasitophorous vacuolar (PV) and is essential for parasite growth. Previous studies suggested that PfPV1 cooperates with the Plasmodium translocon of exported proteins (PTEX) complex to export various proteins from the PV. However, the structure and function of PfPV1 have not been determined in detail. In this study, we undertook the expression, purification, and characterization of PfPV1. The tetramer appears to be the structural unit of PfPV1. The activity of PfPV1 appears to be similar to that of molecular chaperones, and it may interact with various proteins. PfPV1 could substitute CtHsp40 in the CtHsp104, CtHsp70, and CtHsp40 protein disaggregation systems. Based on these results, we propose a model in which PfPV1 captures various PV proteins and delivers them to PTEX through a specific interaction with HSP101.

References

Jun 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·C Raventos-SuarezR L Nagel
Feb 26, 1998·Cell·B Bukau, A L Horwich
Jun 30, 1998·Trends in Biochemical Sciences·W L Kelley
Feb 8, 2000·The Journal of Biological Chemistry·K K SharmaP T Quinn
Jul 28, 2001·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·T Ogura, A J Wilkinson
Dec 14, 2001·The Journal of Biological Chemistry·Yo-hei WatanabeMasasuke Yoshida
Apr 30, 2003·Biochemistry·Pernilla Wittung-StafshedeSamuel J Landry
Apr 30, 2004·Cell Stress & Chaperones·Chun-Yang FanDouglas M Cyr
May 7, 2004·The Journal of Biological Chemistry·Ronnie LumJohn R Glover
Dec 14, 2004·Science·Matthias MartiAlan F Cowman
Jun 19, 2009·Nature·Tania F de Koning-WardBrendan S Crabb
Jul 3, 2013·Annual Review of Microbiology·Justin A Boddey, Alan F Cowman
Oct 1, 2013·Molecular and Biochemical Parasitology·Joachim M MatzTaco W A Kooij
Jul 22, 2014·Nature·Brendan ElsworthTania F de Koning-Ward
Jan 15, 2015·Proceedings of the National Academy of Sciences of the United States of America·K Sony ReddyDeepak Gaur
Jan 27, 2015·Annual Review of Biochemistry·Natalie J SpillmanDaniel E Goldberg
Jul 15, 2015·Frontiers in Molecular Biosciences·Jude M PrzyborskiGregory L Blatch
Mar 22, 2016·Molecular Microbiology·Jude M PrzyborskiMichael Lanzer
Jul 13, 2016·Scientific Reports·Paramita SahaMahua Ghosh
Nov 21, 2016·Biochemical and Biophysical Research Communications·Kazuaki HakamadaMasafumi Yohda

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Citations

Apr 2, 2021·PLoS Pathogens·Josh R Beck, Chi-Min Ho

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Methods Mentioned

BETA
immunoprecipitation
electron microscopy
surface plasmon resonance
Light
size exclusion chromatography
chip
Assay

Software Mentioned

Biacore X100
SEDFIT
Relion

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