PVT1 regulates inflammation and cardiac function via the MAPK/NF-κB pathway in a sepsis model

Experimental and Therapeutic Medicine
Fang FengChaohui Yang


The aim of the present study was to investigate the role of plasmacytoma variant translocation gene 1 (PVT1) in the occurrence and development of sepsis-induced inflammation and cardiac dysfunction and its underlying mechanism. A sepsis rat model was first established by cecal ligation and puncture. The mRNA levels of PVT1 and microRNA-143 in the myocardial tissues of rats were detected by reverse transcription-quantitative polymerase chain reaction (RT-qPCR) analysis. Cardiac function, levels of myocardial injury markers and inflammatory indicators were detected following PVT1 knockdown. The regulatory effect of microRNA-143 on PVT1 was assessed using a luciferase reporter gene assay and RT-qPCR analysis. The specific role of PVT1 in regulating the mitogen-activated protein kinase (MAPK)/nuclear factor (NF)-κB pathway was detected using western blot analysis. PVT1 was downregulated and microRNA-143 was upregulated in the myocardial tissues of sepsis rats. The left ventricular peak pressure was markedly decreased in the sepsis rats. By contrast, the left ventricular end diastolic pressure, levels of inflammatory indicators, myocardial injury markers and complement proteins of C5 and C5a were increased in the sepsis rats. The ab...Continue Reading


Jun 14, 2000·American Journal of Physiology. Heart and Circulatory Physiology·J W HortonB Sanders
Jan 5, 2002·The Journal of Biological Chemistry·Punita Dhawan, Ann Richmond
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Oct 3, 2003·Cardiovascular Research·Jean-Philippe TessierYvan Fischer
Dec 29, 2005·The Journal of Experimental Medicine·Andreas D NiederbichlerPeter A Ward
Jun 15, 2007·Genome Research·Mark B GersteinMichael Snyder
Jul 5, 2008·Critical Care : the Official Journal of the Critical Care Forum·Jean-Louis Vincent, John C Marshall
Aug 5, 2008·Neuroscience·D A Ridder, M Schwaninger
Nov 4, 2008·Acta Paediatrica·Rakesh LodhaSushil K Kabra
Jul 28, 2009·Current Opinion in Critical Care·Sergio L Zanotti-Cavazzoni, Steven M Hollenberg
Jan 19, 2010·Biochimica Et Biophysica Acta·Eun Kyung Kim, Eui-Ju Choi
Mar 10, 2010·Journal of Molecular and Cellular Cardiology·Reut FallachEdith Hochhauser
Nov 12, 2013·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Xiaoxiao WangJingyu Yang
May 20, 2014·JAMA : the Journal of the American Medical Association·Vincent LiuTheodore J Iwashyna
Dec 17, 2015·Nature Reviews. Genetics·Jeffrey J Quinn, Howard Y Chang
Jun 21, 2017·Trends in Genetics : TIG·Huang WuLing-Ling Chen

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