PMID: 9553050May 30, 1998Paper

pX, the HBV-encoded coactivator, suppresses the phenotypes of TBP and TAFII250 mutants

Genes & Development
I HavivY Shaul

Abstract

Hepatitis B virus (HBV) infects humans and causes a wide range of clinical manifestations, from acute hepatitis to hepatocellular carcinoma (HCC). The HBV genome contains multiple promoters with gene expression regulated predominantly by the cellular transcription initiation machinery. Accordingly, the HBV-encoded pX, the only known viral regulator, is a potent transcription coactivator. We investigated the relationship between pX and cellular coactivators. We show that pX restores wild-type activity to inactive TBPAS mutants with poor TAFII250 and activator-binding activity. This pX-mediated recovery, however, is not obtained with inactive TBPAS mutants in binding of other general transcription factors. Remarkably, ts13, a cell line temperature sensitive for TAFII250 function, exhibiting growth arrest and apoptosis at the restrictive temperature, is rescued partially by pX expression, thus generating a pX-dependent cell growth. Collectively, our results suggest that pX suppresses some of the phenotypes of TBP and TAFII250 mutations, implying that pX circumvents the need for a holo-TFIID complex for transcription activation to proceed.

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Citations

Jul 27, 2001·Journal of Medical Virology·J HoareM J McGarvey
Jan 11, 2000·Molecular and Cellular Biology·N ParanY Shaul
Feb 3, 1999·Annual Review of Genetics·L S Rose, K J Kemphues
Nov 15, 2000·Critical Reviews in Clinical Laboratory Sciences·C Rabe, W H Caselmann
Feb 19, 2002·Expert Opinion on Biological Therapy·Lisa H Butterfield, Antoni Ribas
Feb 10, 2016·Proceedings of the National Academy of Sciences of the United States of America·Tianyu JiangYigong Shi
Apr 21, 2001·Virology·O BarakY Shaul
Aug 31, 2004·World Journal of Gastroenterology : WJG·Dan LiQi-Min Tao
Jan 15, 2002·The Journal of Biological Chemistry·Meir ShamayYosef Shaul

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