Abstract
Hepatitis B virus (HBV) infects humans and causes a wide range of clinical manifestations, from acute hepatitis to hepatocellular carcinoma (HCC). The HBV genome contains multiple promoters with gene expression regulated predominantly by the cellular transcription initiation machinery. Accordingly, the HBV-encoded pX, the only known viral regulator, is a potent transcription coactivator. We investigated the relationship between pX and cellular coactivators. We show that pX restores wild-type activity to inactive TBPAS mutants with poor TAFII250 and activator-binding activity. This pX-mediated recovery, however, is not obtained with inactive TBPAS mutants in binding of other general transcription factors. Remarkably, ts13, a cell line temperature sensitive for TAFII250 function, exhibiting growth arrest and apoptosis at the restrictive temperature, is rescued partially by pX expression, thus generating a pX-dependent cell growth. Collectively, our results suggest that pX suppresses some of the phenotypes of TBP and TAFII250 mutations, implying that pX circumvents the need for a holo-TFIID complex for transcription activation to proceed.
References
Sep 1, 1977·Journal of Cellular Physiology·A Talavera, C Basilico
Oct 1, 1992·Genes & Development·Q ZhouA J Berk
Mar 20, 1992·Cell·J D ParvinP A Sharp
May 10, 1991·Science·H F MaguireA Siddiqui
Aug 9, 1991·Cell·B D DynlachtR Tjian
Jul 12, 1990·Nature·K J MartinM R Green
Nov 15, 1995·Genes & Development·S Ruppert, R Tjian
Jun 20, 1995·Proceedings of the National Academy of Sciences of the United States of America·R HoriM Carey
Jun 1, 1995·Experimental Cell Research·T SekiguchiT Nishimoto
Feb 14, 1995·Proceedings of the National Academy of Sciences of the United States of America·I QadriA Siddiqui
Oct 7, 1994·Cell·J L ChenR Tjian
Oct 25, 1994·Proceedings of the National Academy of Sciences of the United States of America·J Benn, R J Schneider
Nov 15, 1994·Genes & Development·W P TanseyW Herr
Dec 1, 1994·Genes & Development·N L HenryR D Kornberg
Sep 12, 1994·FEBS Letters·T De-Medina, Y Shaul
Feb 15, 1994·Virology·S MurakamiS Kaneko
Mar 15, 1994·Proceedings of the National Academy of Sciences of the United States of America·X W WangC C Harris
Apr 20, 1994·Gene·T HayashidaT Nishimoto
Nov 5, 1993·Cell·J A GoodrichR Tjian
Dec 1, 1993·Virology·L RunkelH Schaller
Feb 11, 1994·Science·E H Wang, R Tjian
Jul 1, 1993·Genes & Development·C M TyreeJ T Kadonaga
Sep 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·J C CrossW J Rutter
Sep 1, 1993·Genes & Development·T G Boyer, A J Berk
Feb 25, 1993·Nature·A S KekuléP H Hofschneider
Jun 1, 1996·Genes & Development·B Damania, J C Alwine
Jun 28, 1996·The Journal of Biological Chemistry·I QadriA Siddiqui
Oct 1, 1996·Genes & Development·G O BryantA J Berk
Mar 14, 1997·The Journal of Biological Chemistry·Y LinS Murakami
Mar 14, 1997·The Journal of Biological Chemistry·J P NicolasM H Gelb
Citations
Jul 27, 2001·Journal of Medical Virology·J HoareM J McGarvey
Apr 28, 2001·Cytokine & Growth Factor Reviews·J DiaoC D Richardson
Jul 8, 2009·The Journal of Biological Chemistry·Aya FukudaKoji Hisatake
Jun 16, 2004·Nucleic Acids Research·Kiyohito MuraiNozomu Mori
Feb 13, 2001·Journal of Virology·K V Kibler, K T Jeang
Apr 5, 2001·Journal of Virology·M BouchardR J Schneider
Jan 11, 2000·Molecular and Cellular Biology·N ParanY Shaul
Feb 3, 1999·Annual Review of Genetics·L S Rose, K J Kemphues
Nov 5, 1999·Biochemical and Biophysical Research Communications·D H LeeH M Rho
Nov 15, 2000·Critical Reviews in Clinical Laboratory Sciences·C Rabe, W H Caselmann
Feb 19, 2002·Expert Opinion on Biological Therapy·Lisa H Butterfield, Antoni Ribas
Feb 10, 2016·Proceedings of the National Academy of Sciences of the United States of America·Tianyu JiangYigong Shi
Apr 1, 2004·Molecular Cell·Heng-Hong LiXuan Liu
Apr 21, 2001·Virology·O BarakY Shaul
May 22, 2003·Virology·Gilad Doitsh, Yosef Shaul
Aug 31, 2002·Virology·Marta CarreteroManuel López-Cabrera
Dec 22, 1999·Oncogene·G Doitsh, Y Shaul
Aug 31, 2004·World Journal of Gastroenterology : WJG·Dan LiQi-Min Tao
Jan 15, 2002·The Journal of Biological Chemistry·Meir ShamayYosef Shaul
Jul 18, 2001·The Journal of Biological Chemistry·M Gómez-GonzaloM López-Cabrera
Nov 20, 1998·Molecular and Cellular Biology·H D WangD L Johnson
Aug 24, 1999·Molecular and Cellular Biology·R WeilA Israël
Jan 31, 2002·World Journal of Gastroenterology : WJG·S P GuoY L Zhao