Pαx6 expression in postmitotic neurons mediates the growth of axons in response to SFRP1.

PloS One
Alvaro Sebastián-SerranoMarta Nieto

Abstract

During development, the mechanisms that specify neuronal subclasses are coupled to those that determine their axonal response to guidance cues. Pax6 is a homedomain transcription factor required for the specification of a variety of neural precursors. After cell cycle exit, Pax6 expression is often shut down in the precursor progeny and most postmitotic neurons no longer express detectable levels of the protein. There are however exceptions and high Pax6 protein levels are found, for example, in postmitotic retinal ganglion cells (RGCs), dopaminergic neurons of the olfactory bulb and the limbic system in the telencephalon. The function of Pax6 in these differentiating neurons remains mostly elusive. Here, we demonstrate that Pax6 mediates the response of growing axons to SFRP1, a secreted molecule expressed in several Pax6-positive forebrain territories. Forced expression of Pax6 in cultured postmitotic cortical neurons, which do not normally express Pax6, was sufficient to increment axonal length. Growth was blocked by the addition of anti-SFRP1 antibodies, whereas exogenously added SFRP1 increased axonal growth of Pax6-transfected neurons but not that of control or untransfected cortical neurons. In the reverse scenario, shRN...Continue Reading

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Citations

Jan 15, 2013·Stem Cells International·Ashley McDonough, Verónica Martínez-Cerdeño
Feb 13, 2014·Toxicological Sciences : an Official Journal of the Society of Toxicology·Marie-Claude SenutDouglas M Ruden
Apr 16, 2015·PloS One·Juan Ignacio Díaz-HernándezMaría Teresa Miras-Portugal
Dec 17, 2014·Frontiers in Psychology·Cedric Boeckx, Antonio Benítez-Burraco
Mar 3, 2020·Frontiers in Bioengineering and Biotechnology·Ruchi SharmaStephanie M Willerth
Oct 30, 2020·Journal of Cell Science·Gilbert LauterPeter Swoboda

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Methods Mentioned

BETA
transfection
PCR
phage display
dissecting
mechanical dissociation
fluorescence microscopy

Software Mentioned

LASAF
LaserPix
Win MIDI

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