PYGB siRNA inhibits the cell proliferation of human osteosarcoma cell lines

Molecular Medicine Reports
Shuwei ZhangWei Jin

Abstract

Osteosarcoma is the most common malignant bone carcinoma that primarily occurs between childhood to adolescence. It was suggested by recent research that the Brain type glycogen phosphorylase (PYGB) gene may serve an important role in various types of cancer. In the present study, the PYGB gene was knocked down in order to evaluate the cell viability, invasion and migration of the human osteosarcoma cell lines MG63 and HOS. The expression levels of PYGB in osteosarcoma and bone cyst tissue samples, as well as in the osteosarcoma cell lines were identified using reverse transcription‑quantitative polymerase chain reaction and western blot assay. Subsequently, a Cell Counting kit 8 assay was employed to evaluate cell proliferation. Cell apoptosis rate and cell cycle distribution were measured by flow cytometry. In addition, cell invasion and migration were evaluated through a Transwell assay. The expression levels of the cell apoptosis and tumor metastasis associated proteins B‑cell lymphoma 2 (Bcl‑2), Bcl‑2‑associated X protein, E‑cadherin, Twist, matrix metalloproteinase (MMP)‑9 and MMP2 were measured via western blotting. PYGB exhibited a higher expression level in the osteosarcoma tissue samples, particularly in the human ost...Continue Reading

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Citations

Mar 20, 2020·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Lina XiaoJingyi Zhang
Dec 17, 2020·Frontiers in Cell and Developmental Biology·Guangying CuiShen Shen

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Methods Mentioned

BETA
transfection
gene knockdown
PCR
flow

Software Mentioned

ABI Prism 7300
Accuri C6
ImageJ
GraphPad Prism

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