PYK-2 is tyrosine phosphorylated after activation of pituitary adenylate cyclase activating polypeptide receptors in lung cancer cells.

Journal of Molecular Neuroscience : MN
Terry W MoodyRobert T Jensen

Abstract

The signal transduction mechanisms of pituitary adenylate cyclase activating polypeptide (PACAP) were investigated in lung cancer cells. Previously, PACAP-27 addition to NCI-H838 cells increased phosphatidylinositol turnover and intracellular cAMP leading to proliferation of lung cancer cells. Also, PACAP receptors (PAC1) regulated the tyrosine phosphorylation of ERK, focal adhesion kinase, and paxillin. In this communication, the effects of PACAP on cytosolic Ca(2+) and PYK-2 tyrosine phosphorylation were investigated. PACAP-27 increased cytosolic Ca(2+) within seconds after addition to FURA-2 AM loaded NCI-H838 cells. The increase in cytosolic Ca(2+) caused by PACAP was inhibited by PACAP(6-38) (PAC1 antagonist), U73122 (phospholipase C inhibitor), or BAPTA (calcium chelator), but not H89 (PKA inhibitor). PACAP-38, but not vasoactive intestinal peptide (VIP), addition to NCI-H838 or H1299 cells significantly increased the tyrosine phosphorylation of PYK-2 after 2 min. The increase in PYK-2 tyrosine phosphorylation caused by PACAP was inhibited by PACAP(6-38), U73122, or BAPTA, but not H89. The results suggest that PAC1 regulates PYK-2 tyrosine phosphorylation in a calcium-dependent manner.

References

Jun 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·M D SchallerJ T Parsons
Jan 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·M SchultzeA Kondorosi
Jul 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·J R Pisegna, S A Wank
Oct 27, 2001·Breast Cancer Research and Treatment·T W MoodyI Gozes
Feb 19, 2002·Molecular and Cellular Endocrinology·Antonietta PicasciaDonatella Tramontano
Feb 7, 2003·Current Pharmaceutical Design·Terry W MoodyRobert T Jensen
Feb 11, 2003·Peptides·Terry W MoodyRobert T Jensen
Jun 22, 2005·Neoplasia : an International Journal for Oncology Research·Christopher A LipinskiJoseph C Loftus
Nov 17, 2007·Cancer Research·Jyotsnabaran HalderAnil K Sood
Mar 15, 2008·Cancer Research·Walter Gregory RobertsFelix Vajdos
Jun 24, 2008·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·Siyang ZhangEnhua Wang
Jul 22, 2008·Nature Medicine·UNKNOWN Director's Challenge Consortium for the Molecular Classification of Lung AdenocarcinomaDavid G Beer
Dec 17, 2009·Expert Opinion on Therapeutic Targets·Christopher A Lipinski, Joseph C Loftus
Apr 13, 2011·International Review of Cell and Molecular Biology·Jessica E HallMichael D Schaller
Mar 6, 2012·The Journal of Pharmacology and Experimental Therapeutics·Terry W MoodyRobert T Jensen

❮ Previous
Next ❯

Citations

Feb 19, 2014·Journal of Molecular Neuroscience : MN·Eszter BankiDora Reglodi

❮ Previous
Next ❯

Related Concepts

Related Feeds

Calcium & Bioenergetics

Bioenergetic processes, including cellular respiration and photosynthesis, concern the transformation of energy by cells. Here is the latest research on the role of calcium in bioenergetics.