Pyoverdin-facilitated iron uptake in Pseudomonas aeruginosa: immunological characterization of the ferripyoverdin receptor

Molecular Microbiology
J M MeyerP Cornelis

Abstract

A purified polyclonal antiserum directed against the isolated main 80 kD IROMP (iron-regulated outer-membrane protein) from Pseudomonas aeruginosa PAO1 detected only the 80 kD polypeptide of outer-membrane proteins from PAO1 cells grown in iron deficiency in Western blots. It was also shown to inhibit the uptake of 59Fe pyoverdin by PAO1 cells as well as its binding to purified outer membranes. Immunofluorescence experiments with intact PAO1 cells confirmed that the receptor is present only at the surface of cells grown under conditions of iron deficiency. All these data allow us to conclude that the 80 kD main IROMP of P. aeruginosa is indeed the receptor for the siderophore ferripyoverdin.

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Citations

Jan 1, 1991·Biology of Metals·E Griffiths
Jul 17, 1999·The Journal of Peptide Research : Official Journal of the American Peptide Society·O Kinzel, H Budzikiewicz
Apr 27, 2010·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Seyed Mehdi Larrie BaghalIraj Rasooli
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Jan 1, 1997·Microbiology·Jean-Marie MeyerHerbert Budzikiewicz
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Sep 18, 1997·Microbiology and Molecular Biology Reviews : MMBR·J H Crosa

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