Pyrazinamide resistance among multidrug-resistant tuberculosis clinical isolates in a national referral center of China and its correlations with pncA, rpsA, and panD gene mutations

Diagnostic Microbiology and Infectious Disease
Yunting GuHairong Huang

Abstract

Our study was aimed to identify the phenotypic and genotypic pyrazinamide (PZA) resistance features among multidrug-resistant (MDR) isolates in a national tuberculosis (TB) referral center of China. PZA susceptibility test was performed for a total of 142 MDR-TB clinical isolates using the MGIT 960 PZA kits, and the pncA, rpsA, and panD genes were sequenced. Extensively drug-resistant (XDR) and pre-XDR strains had higher PZA resistance rate than that of MDR strains which were still sensitive to fluoroquinolone and aminoglycoside (42.9%, 24/56) (χ(2)=8.922, P=0.012). No panD mutation was detected among the PZA resistant strains with wild-type pncA and rpsA genes. Our study indicates that PZA-resistant frequency increases with TB drug resistance level; pncA, rpsA, and panD mutations had strong, low, and no correlation with PZA resistance, and rapid molecular assay will facilitate the timely identification of the PZA-sensitive MDR-TB.

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Citations

Apr 16, 2017·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·Y HuH Huang
Oct 6, 2017·Journal of Cellular Biochemistry·Mansi AggarwalAbhinav Grover
Apr 14, 2017·Journal of Clinical Microbiology·Jim WerngrenMikael Mansjö
Jul 31, 2019·Journal of Biomolecular Structure & Dynamics·Muhammad Tahir Khan, Shaukat Iqbal Malik
Sep 14, 2019·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Majid Faridgohar
May 11, 2017·Frontiers in Microbiology·Mohammad J NasiriMohammad M Feizabadi
Mar 7, 2020·Microbiology and Molecular Biology Reviews : MMBR·Elise A LamontAnthony D Baughn
Apr 29, 2020·Journal of Biomolecular Structure & Dynamics·Muhammad JunaidDong-Qing Wei
Oct 31, 2020·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Sajid AliMuhammad Irfan
Apr 17, 2021·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Muhammad Tahir KhanDong Qing Wei
Nov 28, 2018·Journal of Chemical Information and Modeling·Muhammad JunaidDong-Qing Wei
Jun 1, 2018·Journal of Medicinal Chemistry·Rupesh V ChikhaleMange Ram Yadav
Sep 29, 2021·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Mohammad BagheriMohammad Javad Nasiri

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