PMID: 9531487Jun 11, 1998Paper

Pyrazole-inducible proteins in DBA/2 mouse liver bind with high affinity to the 3'-untranslated regions of the mRNAs of coumarin hydroxylase (CYP2A5) and c-jun

The Biochemical Journal
M Thulke-GrossH Bartsch

Abstract

An important mechanism in the up-regulation of cytochrome P-450 2A5 (CYP2A5, coumarin hydroxylase, Coh) is the stabilization of the corresponding mRNA; some evidence suggests that proteins binding to CYP2A5 mRNA may be involved in this stabilization. Here we report that pyrazole, a well known inducer of CYP2A5 and stabilizer of its message, enhances the binding of a set of proteins to 32P-labelled 3'-untranslated region (3'UTR) of CYP2A5 to give 32P-labelled bands of apparent molecular mass 37/39, 45/48 and 70/72 kDa after UV cross-linking/RNase cleavage; in addition, we found different proteins binding to other parts of CYP2A5 mRNA. The 70/72 kDa bands are also formed with the 3'UTR of c-jun. The inducible proteins are found in different cellular subfractions at different concentrations, with a maximum of five-fold induction of binding activity in microsomes. When a gel-mobility-shift assay was combined with UV cross-linking to resolve different pyrazole-inducible RNA-protein complexes into single RNA-binding protein bands, the smallest complex contained a double band of 37/39 kDa, 45/48 kDa bands, 70/72 kDa bands, and additional weaker bands at higher molecular masses (around 120 kDa). This composition was found also for all ...Continue Reading

Citations

Apr 30, 2002·Journal of Biochemical and Molecular Toxicology·John M SeubertJohn R Bend
Dec 25, 2004·The Biochemical Journal·Nhu-Traï TruongIsabelle de Waziers
Sep 25, 2002·The Journal of Biological Chemistry·Arlette MoncionIsabelle De Waziers
Oct 21, 2003·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Elena PirasEva B Brittebo
May 1, 2013·Toxicology and Applied Pharmacology·Sangsoo Daniel KimGordon M Kirby
May 12, 2004·Chemico-biological Interactions·Guoyu LingXinxin Ding

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