Pyrene-stacked nanostructures constructed in the recombinant tobacco mosaic virus rod scaffold

Chemistry : a European Journal
Masayuki EndoTetsuro Majima

Abstract

The effect of pyrenes introduced into a tobacco mosaic virus (TMV) coat protein monomer on the formation and stability of the TMV assembly was investigated. The possible arrangement of the pyrenes in the inner cavity of the TMV rod was also estimated. The pyrene derivative was introduced to four specific amino acids in the cavity of the TMV rod structure. Rod-structure formation was examined by atomic force microscopy (AFM). Two pyrene-attached mutants (positions 99 and 100) assembled to increase the length of the rod structures by 2.5 microm at pH 5.5. The interaction of the pyrene moieties in the TMV cavity was investigated by steady-state and time-resolved spectroscopic analysis. Strong excimer emission with significantly short wavelength (465 nm) was observed from the two mutants mentioned above. Excitation and UV-visible spectra indicate that the pyrene moieties form pi-stacked structures in the TMV cavity. Details of the pyrene interaction were investigated by analyzing the fluorescence lifetime of the excimer. Results suggest that the pyrenes formed preassociated rigid structures with partially overlapped geometry in the restricted space of the TMV cavity. The pyrenes effectively stabilize the TMV rod through a pi-stacki...Continue Reading

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Citations

Mar 19, 2013·Journal of Virological Methods·Anan KadriHolger Jeske
Sep 15, 2011·Biotechnology and Bioengineering·Sang-Yup LeeMichael T Harris
Sep 13, 2007·Chemistry : a European Journal·Masayuki EndoTetsuro Majima
Apr 27, 2010·Chemical Society Reviews·Erik SchwartzAlan E Rowan
Nov 9, 2012·Organic & Biomolecular Chemistry·Mitsunobu NakamuraKazushige Yamana
Jul 10, 2014·Chemical Communications : Chem Comm·Quan LuoJunqiu Liu
May 20, 2017·Bioinspiration & Biomimetics·Kannan Badri Narayanan, Sung Soo Han
Sep 7, 2015·Journal of Materials Chemistry. B, Materials for Biology and Medicine·D VilonaM Bonchio
Nov 16, 2010·Angewandte Chemie·Kazunori MatsuuraNobuo Kimizuka
Oct 15, 2008·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·Yue ZhaoJiyu Fang
Apr 16, 2010·Journal of the American Chemical Society·Rebekah A MillerMatthew B Francis
Mar 3, 2011·ACS Nano·Michael A BruckmanAmy Szuchmacher Blum
Feb 27, 2007·Journal of the American Chemical Society·Rebekah A MillerMatthew B Francis
Apr 13, 2007·Bioconjugate Chemistry·Hannah N BarnhillQian Wang

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