PMID: 12379355Oct 16, 2002

Pyridoxal-5'-phosphate-dependent catalytic antibodies

Journal of Immunological Methods
S GramatikovaPhilipp Christen


Strategies for expanding the catalytic scope of antibodies include the incorporation of inorganic or organic cofactors into their binding sites. An obvious choice is pyridoxal-5'-phosphate (PLP), which is probably the most versatile organic cofactor of enzymes. Monoclonal antibodies against the hapten N(alpha)-(5'-phosphopyridoxyl)-L-lysine, a stable analog of the covalent coenzyme-substrate adducts were screened by a competition ELISA for binding of the PLP-amino acid Schiff base adduct. The Schiff base with its C4'-N alpha double bond is, in contrast to the hapten, a planar compound and is an obligatory intermediate in all PLP-dependent reactions of amino acids. This highly discriminating screening step eliminated all but 5 of 24 hapten-binding antibodies. The five remaining antibodies were tested for catalysis of the PLP-dependent alpha,beta-elimination reaction of beta-chloroalanine. Antibody 15A9 complied with this selection criterion and catalyzed in addition the cofactor-dependent transamination reaction of hydrophobic D-amino acids and oxo acids (k(cat)'=0.42 min(-1) with D-alanine at 25 degrees C). Homology modeling together with alanine scanning yielded a 3D model of Fab 15A9. The striking analogy between antibody 15A...Continue Reading


Jul 5, 1992·Journal of Molecular Biology·J B MitchellS L Price
Jan 5, 1991·Journal of Molecular Biology·I S MianA J Olson
Apr 1, 1966·Proceedings of the National Academy of Sciences of the United States of America·H C Dunathan
Apr 15, 1984·Journal of Molecular Biology·J F KirschPhilipp Christen
Apr 27, 1993·Biochemistry·A A AntsonK S Wilson
Aug 2, 1994·Proceedings of the National Academy of Sciences of the United States of America·J D StewartS J Benkovic
Feb 23, 1996·Science·P A PattenP G Schultz
Nov 29, 1996·The Journal of Biological Chemistry·S Gramatikova, Philipp Christen
Aug 29, 1997·The Journal of Biological Chemistry·R A VaccaPhilipp Christen
May 21, 1998·Nature Biotechnology·I FujiiR Tanimura
Jan 23, 1999·Current Opinion in Structural Biology·J N Jansonius
Nov 30, 2000·Proceedings of the National Academy of Sciences of the United States of America·A V KolesnikovA Friboulet
Apr 5, 2002·Chemical Record : an Official Publication of the Chemical Society of Japan ... [et Al.]·Philipp Christen, P K Mehta


Dec 28, 2005·Bioorganic & Medicinal Chemistry·Rachel L ClarkSimon P Mackay
Oct 17, 2008·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Fang Wu, Heinz Gehring
Jun 23, 2006·The Journal of Biological Chemistry·Béatrice Golinelli-PimpaneauPhilipp Christen

Related Concepts

Fab Immunoglobulins
Pyridoxal Phosphate
Antibodies, Catalytic
Tertiary Protein Structure

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Synthetic Genetic Array Analysis

Synthetic genetic arrays allow the systematic examination of genetic interactions. Here is the latest research focusing on synthetic genetic arrays and their analyses.

Congenital Hyperinsulinism

Congenital hyperinsulinism is caused by genetic mutations resulting in excess insulin secretion from beta cells of the pancreas. Here is the latest research.

Neural Activity: Imaging

Imaging of neural activity in vivo has developed rapidly recently with the advancement of fluorescence microscopy, including new applications using miniaturized microscopes (miniscopes). This feed follows the progress in this growing field.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Epigenetic Memory

Epigenetic memory refers to the heritable genetic changes that are not explained by the DNA sequence. Find the latest research on epigenetic memory here.

Cell Atlas of the Human Eye

Constructing a cell atlas of the human eye will require transcriptomic and histologic analysis over the lifespan. This understanding will aid in the study of development and disease. Find the latest research pertaining to the Cell Atlas of the Human Eye here.

Femoral Neoplasms

Femoral Neoplasms are bone tumors that arise in the femur. Discover the latest research on femoral neoplasms here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.