Pyrimidine nucleotidases from human erythrocyte possess phosphotransferase activities specific for pyrimidine nucleotides

FEBS Letters
Adolfo AmiciS Ruggieri

Abstract

Two cytoplasmic forms of pyrimidine nucleotidase (PN-I and PN-II) have been purified from human erythrocytes to apparent homogeneity and partially characterized. They preferentially hydrolyse pyrimidine 5'-monophosphates and 3'-monophosphates respectively. PN-I and PN-II operate as interconverting activities, capable of transferring the phosphate from the pyrimidine nucleoside monophosphate donor(s) to various nucleoside acceptors, including important drugs like 3'-azido-3'-deoxy-thymidine (AZT), cytosine-beta-D-arabinofuranoside (AraC) and 5-fluoro-2'-deoxy-uridine (5FdUrd), pyrimidine analogues widely used in chemotherapy. Kinetic analysis showed linear behaviour for both PN-I and PN-II. PN-I phosphotransferase activity revealed higher affinity for oxynucleosides with respect to deoxy-nucleosides, whereas the contrary seems to be true for PN-II. These results show for the first time that soluble pyrimidine nucleotidases are endowed with pyrimidine-specific phosphotransferase activity.

References

Jan 1, 1991·Advances in Experimental Medicine and Biology·A AmiciG Magni
May 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·A De FloraU Benatti
Feb 15, 1988·Clinica Chimica Acta; International Journal of Clinical Chemistry·H A SimmondsE H Harley
Oct 1, 1974·The Journal of Clinical Investigation·W N ValentineW S Adams
Sep 1, 1982·The Biochemical Journal·Y Worku, A C Newby
Jan 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·D E PagliaR A Brockway

❮ Previous
Next ❯

Citations

Mar 9, 2007·Cancer Metastasis Reviews·Jing ZhangCarol E Cass
Sep 6, 2007·Journal of Structural and Functional Genomics·George N PhillipsSimon N Twigger
Jun 21, 2005·Pharmacology & Therapeutics·Sally Anne HunsuckerJozef Spychala
Feb 14, 2002·Archives of Biochemistry and Biophysics·Adolfo Amici, Giulio Magni
Apr 14, 2006·British Journal of Haematology·Alberto ZanellaGiovanna Valentini
Feb 13, 2003·British Journal of Haematology·David C ReesAnthony M Marinaki
Aug 29, 2012·Journal of Molecular Biology·Christina L GroboskyEduard Bitto
Dec 29, 2004·Leukemia & Lymphoma·Paula Fernandez-CalottiCarlos Maria Galmarini
Jul 4, 2001·The Journal of Biological Chemistry·S AllegriniM G Tozzi
Feb 25, 2009·Expert Opinion on Pharmacotherapy·Michael J Absalon, Franklin O Smith
Aug 30, 2003·The Journal of Biological Chemistry·Vera Bianchi, Jozef Spychala
May 5, 2006·The Journal of Biological Chemistry·Eduard BittoGeorge N Phillips

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.