Pyrimidine nucleotide metabolism and pathways of thymidine triphosphate biosynthesis in Salmonella typhimurium.

Journal of Bacteriology
J Neuhard

Abstract

The nucleoside triphosphate pools of two cytidine auxotrophic mutants of Salmonella typhimurium LT-2 were studied under different conditions of pyrimidine starvation. Both mutants, DP-45 and DP-55, are defective in cytidine deaminase and cytidine triphosphate (CTP) synthase. In addition, DP-55 has a requirement for uracil (uridine). Cytidine starvation of the mutants results in accumulation of high concentrations of uridine triphosphate (UTP) in the cells, while the pools of CTP and deoxy-CTP drop to undetectable levels within a few minutes. Addition of deoxycytidine to such cells does not restore the dCTP pool, indicating that S. typhimurium has no deoxycytidine kinase. From the kinetics of UTP accumulation during cytidine starvation, it is concluded that only cytidine nucleotides participate in the feedback regulation of de novo synthesis of UTP; both uridine and cytidine nucleotides participate in the regulation of UTP synthesis from exogenously supplied uracil or uridine. Uracil starvation of DP-55 in presence of cytidine results in extensive accumulation of CTP, suggesting that CTP does not regulate its own synthesis from exogenous cytidine. Analysis of the thymidine triphosphate (dTTP) pool of DP-55 labeled for several ge...Continue Reading

Citations

Jan 1, 1989·Archives of Microbiology·G A O'DonovanP K Dutta
Nov 11, 1971·European Journal of Biochemistry·W Uerkvitz
Jun 8, 1977·Molecular & General Genetics : MGG·C Janion
Jul 14, 1971·Nature: New Biology·P P Dennis
May 8, 2002·The Journal of Experimental Medicine·F Nina Papavasiliou, David G Schatz
Jan 1, 1972·Molecular & General Genetics : MGG·S I Ahmad, R H Pritchard
May 21, 2003·The Journal of Experimental Medicine·Sarah K DickersonF Nina Papavasiliou
Jan 1, 1971·Molecular & General Genetics : MGG·C F Beck, J L Ingraham
May 1, 1990·Antonie van Leeuwenhoek·C P Chu, T P West
Feb 24, 2021·ELife·Maarten De Jong, Neal M Alto
Mar 1, 1973·Applied Microbiology·J T Wachsman, D D Morgan
Apr 1, 1971·Journal of Bacteriology·J Hoffmeyer, J Neuhard
Apr 1, 1972·Journal of Bacteriology·C F BeckE Thomassen
Sep 1, 1973·Journal of Bacteriology·J C Williams, G A O'Donovan
Apr 1, 1970·Journal of Bacteriology·P P Dennis, R K Herman
Jan 1, 1993·Antimicrobial Agents and Chemotherapy·S J SperberM P Weinstein
Nov 24, 1982·Biochimica Et Biophysica Acta·T P WestG A O'Donovan
Jan 1, 1970·Biochimica Et Biophysica Acta·G A O'Donovan
Mar 19, 1970·Biochimica Et Biophysica Acta·W Firshein, P Hasselbacher
Mar 1, 1979·Journal of Bacteriology·A Mitchell, L R Finch
Apr 10, 1974·Biochimica Et Biophysica Acta·D Bersier, R Braun
Dec 1, 1974·Journal of Bacteriology·M De FeliceM Iaccarino
May 1, 1975·Journal of Bacteriology·R H Hoess, R K Herman
Mar 1, 1971·Journal of Bacteriology·J T Wachsman, D D Morgan
Sep 1, 2008·EcoSal Plus·Kaj Frank JensenMartin WillemoËs
Jul 1, 1982·Journal of Bacteriology·L B Bussey, J L Ingraham

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.