Pyrimidine nucleotide synthesis in the emerging pathogen Pseudomonas monteilii

Canadian Journal of Microbiology
Jayendra Chunduru, Thomas P West

Abstract

Regulation of pyrimidine biosynthesis by pyrimidines in the emerging, opportunistic human pathogen Pseudomonas monteilii ATCC 700476 was evident. When wild-type cells were grown on succinate in the presence of uracil or orotic acid, the activities of all 5 pyrimidine biosynthetic enzymes were depressed while the activities of 3 of the enzymes decreased in glucose-grown cells supplemented with uracil or orotic acid compared with unsupplemented cells. Pyrimidine limitation of succinate- or glucose-grown pyrimidine auxotrophic cells lacking orotate phosphoribosyltransferase activity resulted in more than a doubling of the pyrimidine biosynthetic enzyme activities relative to their activities in uracil-grown cells. Independent of carbon source, pyrimidine-limited cells of the pyrimidine auxotrophic cells deficient for dihydroorotase activity generally resulted in a slight elevation or depression of the pyrimidine biosynthetic enzyme activities compared with their activities in cells grown under saturating uracil conditions. Aspartate transcarbamoylase activity in P. monteilii was regulated at the enzyme activity level, since the enzyme was strongly inhibited by CTP, UMP, GMP, GDP, ADP, and UTP. In summary, the regulation of pyrimid...Continue Reading

References

Feb 1, 1976·Journal of General Microbiology·S CondonG A O'donovan
Jul 1, 1997·International Journal of Systematic Bacteriology·M ElomariH Leclerc
Aug 12, 2000·International Journal of Systematic and Evolutionary Microbiology·Y AnzaiH Oyaizu
Mar 7, 2003·Canadian Journal of Microbiology·Manuel F Santiago, Thomas P West
Dec 13, 2006·Extremophiles : Life Under Extreme Conditions·Midori MasudaAkira Inoue
Oct 3, 2007·Journal of Basic Microbiology·Thomas P West
Jan 13, 2010·Antonie van Leeuwenhoek·Thomas P West
Jul 19, 2011·The Journal of Antimicrobial Chemotherapy·Pierre BogaertsYouri Glupczynski
Jul 31, 2012·Journal of Bacteriology·Qiao MaPing Xu
Feb 9, 2013·Research in Microbiology·Magdalena MuletJorge Lalucat
May 7, 2014·Marine Genomics·Yang LiuRunying Zeng
Nov 26, 2014·Antimicrobial Agents and Chemotherapy·Alain A Ocampo-SosaLuis Martínez-Martínez
Mar 31, 2015·Bioresource Technology·Morten S DueholmJeppe Lund Nielsen
Jul 26, 2017·BMC Infectious Diseases· AditiKiran Beri

❮ Previous
Next ❯

Citations

Mar 4, 2020·Archives of Microbiology·Anvesh Domakonda, Thomas P West

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.