Pyrogallol red-vanadium complex-a new stain for electron microscopy

Histochemistry and Cell Biology
W VölkerH Robenek

Abstract

We report on the application of a pyrogallol red-vanadium complex (PR-V) for ultracytochemical staining of proteinaceous structures in animal tissues and cell cultures. This dye may be used as a general purpose stain in electron microscopy. In contrast to osmium tetroxide, the price of the material is low and no toxic vapors are produced. The PR-V complex was prepared by addition of vanadium (IV) oxide sulfate to pyrogallol red dissolved in acetate buffer (pH 5.6). The formation of the complex was indicated by a color change from purple-red (lambda max = 520 nm) to violet (lambda max = 539 nm) which occurred at equimolar concentrations of the dye and the metal salt. Under these conditions PR-V was stable for several days. The mechanism of PR-V binding was checked in dot blots using different proteins as well as heparin for control. While heparin remained unstained, proteins were stained in a dose-dependent manner. Deamination of proteins with nitric oxide strongly reduced PR-V staining in dot blots as well as in cell cultures. Optimal staining results of animal cells and tissues were obtained in specimens that had been mildly fixed for at least 1 h or longer with a mixture of 0.1% glutaraldehyde and 1.0% paraformaldehyde dissol...Continue Reading

References

Jul 1, 1972·The Histochemical Journal·D Hopwood
Jan 1, 1969·Journal of Ultrastructure Research·A R Spurr
Oct 1, 1955·Experimental Cell Research·G F BAHR
Jul 25, 1958·The Journal of Biophysical and Biochemical Cytology·M L WATSON

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Citations

Jun 10, 1998·Clinica Chimica Acta; International Journal of Clinical Chemistry·P VoirolP Baumann
Mar 21, 2008·Pediatric Surgery International·Banu TopalogluFerit Bernay

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