Pyroglutamate aminopeptidase 1 may be an indicator of cellular inflammatory response as revealed using a sensitive long-wavelength fluorescent probe

Chemical Science
Qiuyu GongHuimin Ma

Abstract

Pyroglutamate aminopeptidase 1 (PGP-1) can remove pyroglutamic acid from the N-terminus of a polypeptide, including some important anti-inflammatory proteins. Detecting the change and distribution of cellular PGP-1 in an inflammation process would be helpful to better understand the role of this enzyme. However, no report has been found on this subject, mainly due to the lack of a proper research approach. Herein, we develop such a new method by preparing a sensitive long-wavelength fluorescent probe combined with confocal fluorescence imaging. The probe, consisting of l-pyroglutamic acid and cresyl violet, exhibits high selectivity and sensitivity for PGP-1 under physiological conditions. With this probe, the up-regulation of PGP-1 in LO-2 cells under the stimulation of Freund's incomplete adjuvant and lipopolysaccharide (two main immunopotentiators) is revealed for the first time, and this up-regulation is also observed in typical phagocytic RAW264.7 cells, as evidenced by western blot and inhibition assays. Studies on the distribution of PGP-1 in cells using our probe showed that most PGP-1 is located in the cytoplasm, which is further supported by an immunofluorescence assay. Moreover, the inflammatory response induced by t...Continue Reading

References

Dec 20, 1999·Journal of Agricultural and Food Chemistry·Y SuzukiK Sato
Jan 24, 2006·International Immunopharmacology·Camila Eduardo MarinhoPaulo Flavio Silveira
Apr 4, 2006·Analytical Chemistry·Dirk CheliusPavel V Bondarenko
Jan 20, 2009·Biological Psychiatry·Andrew H MillerCharles L Raison
Aug 20, 2009·Current Medicinal Chemistry·E Esposito, S Cuzzocrea
Feb 2, 2011·Cell Research·Matthew T Sorbara, Stephen E Girardin
Jul 19, 2012·Angewandte Chemie·Chung-Hang LeungDik-Lung Ma
Dec 4, 2012·Chemical Communications : Chem Comm·Qiongqiong WanHuimin Ma
May 11, 2013·The International Journal of Biochemistry & Cell Biology·Dereck E W ChattertonPer Torp Sangild
Jul 3, 2013·Langmuir : the ACS Journal of Surfaces and Colloids·Xiangsheng LiuJian Ji
Nov 5, 2014·Accounts of Chemical Research·Dik-Lung MaChung-Hang Leung
Dec 15, 2015·Nature Reviews. Rheumatology·George D Kalliolias, Lionel B Ivashkiv
Jan 21, 2016·Nature·Larry L LuchsingerHans-Willem Snoeck
Feb 2, 2015·Chemical Science·Chung-Hang LeungDik-Lung Ma
Feb 1, 2016·Chemical Science·Mariusz Skwarczynski, Istvan Toth

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Citations

Dec 7, 2018·Chemical Society Reviews·Junji ZhangHe Tian
Nov 3, 2016·Small·Xin LiJun Xie
Mar 14, 2018·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Jie XingAiguo Wu
Aug 25, 2018·Chemical Society Reviews·Hong-Wen LiuWeihong Tan
Apr 22, 2021·Bioorganic & Medicinal Chemistry Letters·Shilong HuMinyong Li
Apr 20, 2021·Methods and Applications in Fluorescence·Asanka Amith RajapakshaGuang-Fu Yang
May 29, 2021·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Ting CaoYun Liu
Jun 23, 2021·Analytical Methods : Advancing Methods and Applications·Jinlei YueWeili Zhao
Jun 28, 2019·Accounts of Chemical Research·Xiaofeng WuHuimin Ma
Dec 4, 2021·Neurotherapeutics : the Journal of the American Society for Experimental NeuroTherapeutics·Anneke KuipersBarbara Kofler

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Methods Mentioned

BETA
fluorescence imaging
Fluorescence
NMR

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