Pyroglutamyl peptidase I and prolyl endopeptidase in human semen: increased activity in necrozoospermia

Regulatory Peptides
Asier ValdiviaLuis Casis

Abstract

Thyrotropin-releasing hormone (TRH) and its analogues have been reported to have important functions in human semen. In the present paper, we have characterized the activity of the TRH-degrading enzymes pyroglutamyl peptidase I and prolyl endopeptidase in the fluid and prostasomes of human semen and in subcellular fractions of the corresponding sperm. Enzymatic activities were measured fluorimetrically using beta-naphthylamine derivatives as substrate. Activity associated with both enzymes was detected in seminal fluid and in the prostasome fraction, as well as in soluble and particulate sperm subcellular fractions. Pyroglutamyl-peptidase I activity presented highest levels in the particulate sperm fraction, whereas the activity of prolyl endopeptidase was maximal in the soluble sperm fraction. In addition, we compared the activity of both enzymes in different seminal fractions in normozoospermic, fertile men and in subfertile patients with different abnormalities revealed by spermiogram analysis (astenozoospermia, necrozoospermia and teratozoospermia). The activities of pyroglutamyl peptidase I and prolyl endopeptidase in necrozoospermia were found to be higher in the corresponding soluble and particulate sperm fractions, resp...Continue Reading

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Citations

Sep 12, 2008·Reproduction : the Official Journal of the Society for the Study of Fertility·Camila Eduardo MarinhoPaulo Flavio Silveira
Feb 20, 2009·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Camila Eduardo MarinhoPaulo Flavio Silveira
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