PMID: 1276Jan 2, 1976

Pyrophosphatase and glucuronosyltransferase in microsomal UDPglucuronic-acid metabolism in the rat liver

European Journal of Biochemistry
E Puhakainen, O Hänninen

Abstract

1. A radiochemical method for the studies on the microsomal UDPglucuronic acid metabolism has been developed. 2. The rat liver microsomes caused a rapid hydrolysis of UDPglucuronic acid to D-glucuronic acid 1-phosphate and further although much slower to free D-glucuronic acid. In Tris-HCl buffer (pH 7.4) they were produced in ratio 72 : 1. No other metabolites were found in measurable amounts. The pyrophosphatase splitting UDPglucuronic acid showed a pH optimum at 8.9, but the liberation of D-glucuronic acid from UDPglucuronic acid had two pH maxima (pH 3.5 and 8.5). EDTA appeared to be less powerful inhibitor of pyrophosphatase than previously suggested. About 25 per cent of the UDPglucuronic acid hydrolyzing activity was still remaining in the presence of 10 mM EDTA. D-Glucaro-1,4-lactone was found to have a slight inhibitory action on the pyrophosphatase activity. Citrate inhibited powerfully the hydrolysis of UDPglucuronic acid and the liberation of free D-glucuronic acid. Phosphate was also inhibitory. 3. In the presence of an exogenous UDPglucuronosyltransferase substrate, 4-nitrophenol, the formation of D-glucuronic acid 1-phosphate and free D-glucuronic acid were slightly reduced, and D-glucuronic acid 1-phosphate, 4-n...Continue Reading

References

Jun 1, 1973·FEBS Letters·J Marniemi, O Hänninen
Oct 14, 1970·Biochimica Et Biophysica Acta·K P Wong, Y K Lau
Jan 1, 1970·Biochimica Et Biophysica Acta·J Skidmore, E G Trams
Feb 15, 1974·FEBS Letters·E Puhakainen, O Hänninen
Jun 1, 1958·Biochimica Et Biophysica Acta·V GINSBURGE S MAXWELL
Jul 1, 1960·Biochimica Et Biophysica Acta·B M POGELL, C R KRISMAN
Sep 9, 1950·Nature·W E TREVELYANJ S HARRISON

Citations

Mar 17, 2009·BMC Systems Biology·Seongwon Seo, Harris A Lewin
Jan 1, 1977·Drug Metabolism Reviews·R R Brenner
May 13, 2006·The FEBS Journal·Carole L Linster, Emile Van Schaftingen
Jan 16, 2007·The FEBS Journal·Carole L Linster, Emile Van Schaftingen
Nov 1, 1981·British Journal of Clinical Pharmacology·E J AntalI R Lawson
Jul 1, 1983·Journal of Clinical Pharmacology·V P GotzA J Block
Apr 1, 1980·Journal of Clinical Pharmacology·S T OmayeH E Sauberlich
Jul 17, 2003·The Journal of Biological Chemistry·Carole L Linster, Emile Van Schaftingen
Mar 25, 2005·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Annalise Di MarcoRalph Laufer
Sep 1, 1984·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·H T CuypersP L Jansen

Related Concepts

Edetic Acid, Sodium Salt
Hexosyltransferase
Hydrogen-Ion Concentration
Microsomes, Liver
Pyrophosphatase
UDP-Glucuronic Acid 3-O-beta-D-Galactosyl-D-Galactose Glucuronosyltransferase
Uridine Diphosphate Glucuronic Acid
Uridine Diphosphate Sugars

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