Pyrosequencing investigation into the influence of Cu2+ , Zn2+ , Fe2+ and I- mixtures on fungal diversity and toxigenic fungal growth in a fermented liquid feed

Animal Nutrition
Yuyong HeHuadong Wu

Abstract

A L9(34) orthogonal experiment was conducted to evaluate the influence of 9 mixtures which consisted of Cu2+, Zn2+, Fe2+ and I- ions at different ion concentrations on fungal diversity and toxigenic fungal growth in a Bacillus subtilis-fermented liquid feed (FLF) using pyrosequencing. The maximal Chao estimator and Shannon index were achieved in the FLF with a mixture of Cu2+ (200 mg/kg), Zn2+ (160 mg/kg), Fe2+ (150 mg/kg) and I- (2.4 mg/kg). The minimal relative abundance of Aspergillus was achieved when a mixture of Cu2+ (200 mg/kg), Zn2+, Fe2+ and I- was added to the FLF. Compared with Zn2+, Fe2+ and I-, Cu2+ was the most important ion in inhibiting Aspergillus growth. Adding Zn2+ (160 mg/kg), Cu2+, Fe2+ and I- to the FLF minimized the relative abundance of Fusarium. It was Zn2+ instead of Cu2+ played a critical role in suppressing the growth of Fusarium. The proper use of the mixture of Cu2+, Zn2+, Fe2+ and I- in FLF contributes to inhibit the growth of mycotoxin-producing fungi during storage. The new findings of this study help farmers properly use the mixture of Cu2+, Zn2+, Fe2+ and I- to inhibit the growth of mycotoxin-producing fungi in the production of high quality FLF and alleviate mycotoxins damages to animals and ...Continue Reading

Datasets Mentioned

BETA
SRP044186

Methods Mentioned

BETA
PCR

Software Mentioned

Mothur
Qiime
R
Usearch

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