PMID: 7542319Jan 1, 1995Paper

Pyrostatins A and B, new inhibitors of N-acetyl-beta-D-glucosaminidase, produced by Streptomyces sp. SA-3501

Journal of Enzyme Inhibition
T AoyamaT Aoyagi

Abstract

Pyrostatins A and B, new inhibitors of N-acetyl-beta-D-glucosaminidase (GlcNAc-ase), have been purified from the culture broth of Streptomyces sp. SA-3501 isolated from a marine environment. They were purified by chromatography on Dowex 50W, silica gel and Capcell Pak C18 (HPLC) followed treatment with active carbon and then isolated as white powders. The structures of pyrostatins A and B were determined by NMR studies to be 4-hydroxy-2-imino-1-methylpyrrolidine-5-carboxylic acid and 2-imino-1-methylpyrrolidine-5-carboxylic acid, respectively. They were competitive with the substrate, and the inhibition constants (Ki) of pyrostatins A and B were 1.7 x 10(-6) M and 2.0 x 10(-6) M respectively.

References

Aug 1, 1977·The Journal of Antibiotics·G A EllestadJ E Lancaster
Jul 1, 1975·The Journal of Antibiotics·T AoyagiT Takeuchi
Sep 1, 1992·The Journal of Antibiotics·T AoyamaT Takeuchi
Sep 1, 1987·The Journal of Antibiotics·M TakizawaT Hasegawa
Jan 1, 1983·Leukemia Research·H G Drexler, G Gaedicke
Apr 1, 1982·Biochemical Medicine·J A Alhadeff, R T Holzinger

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Citations

Jun 27, 2009·Journal of Enzyme Inhibition and Medicinal Chemistry·Daniel Appel, Georg Lentzen
Apr 30, 2019·Chemistry : a European Journal·Hedda Schrey, Peter Spiteller
Dec 1, 2008·Indian Journal of Microbiology·Renu SolankiRup Lal
Nov 7, 2012·Extremophiles : Life Under Extreme Conditions·Javad HamediAntonio Ventosa
Feb 6, 2008·Natural Product Reports·John W BluntMichèle R Prinsep
Apr 16, 1998·Pharmacology & Therapeutics·M HorschD M Rast

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