Abstract
We investigated whether interleukin-1alpha (IL-1) would induce gene expression of vascular cell adhesion molecule-1 (VCAM-1) in cardiac myocytes and, if so, whether nuclear factor kappaB (NF-kappaB) was involved. We evaluated the VCAM-1 gene expression in cultured neonatal rat cardiac myocytes by the reverse transcription-polymerase chain reaction. IL-1 alone or together with interferon-gamma (IFN) induced VCAM-1 gene expression in the cells. Induction of VCAM-1 gene expression in response to IL-1 and IFN was antagonized in a concentration-dependent manner by pyrrolidine dithiocarbamate, an inhibitor of NF-kappa b activation (25-100 microM). Tosyl-lysine-chloromethyl ketone, another inhibitor of NF-kappa b activation, also inhibited the expression of the VCAM-1 gene in response to IL-1 and IFN. Thus, VCAM-1 gene expression appeared to be mediated by NF-kappa b in cardiac myocytes, and this cardiac myocyte VCAM-1 may be involved in cardiac inflammatory disorders. Disruption of expression of VCAM-1 by inhibition of NF-kappa b activation may indicate a target for pharmacologic intervention intended to limit cardiac inflammation.
Citations
Mar 4, 2000·American Journal of Hypertension·N KobayashiH Matsuoka
Feb 24, 2001·Antioxidants & Redox Signaling·S Fujii, T Yoshimura
Aug 3, 2001·Journal of Cardiovascular Pharmacology·S HorinakaH Matsuoka
Apr 21, 2010·Immunology and Cell Biology·Marina KaterelosDavid A Power
Aug 13, 1999·The American Journal of Physiology·N ChoeE Kagan
May 8, 2004·Antioxidants & Redox Signaling·Tetsuhiko Yoshimura, Yashige Kotake
Dec 7, 2005·Journal of Hypertension·Vito De Gennaro ColonnaGiuseppe Rossoni
Apr 25, 2000·Journal of Cardiovascular Pharmacology·T HigashiH Matsuoka
Jun 16, 2006·Journal of Cardiovascular Pharmacology·Shigeo HorinakaHiroaki Matsuoka
Jan 1, 2010·International Journal of Molecular Sciences·David A TiangcoBarbara Y Hargrave
Jul 18, 2002·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Christopher D RaeburnXianzhong Meng
Jan 26, 2018·Journal of Cardiovascular Development and Disease·Nathan A HollandDavid A Tulis
Aug 13, 1999·Journal of Cardiovascular Pharmacology·N KobayashiH Matsuoka
Apr 11, 2002·Chemical Reviews·Tetsuo Nagano, Tetsuhiko Yoshimura
Sep 6, 2003·International Journal of Cardiology·Shigeo HorinakaHiroaki Matsuoka