Pyruvate alleviates high glucose-induced endoplasmic reticulum stress and apoptosis in HK-2 cells.

FEBS Open Bio
Xiao Meng ZhangHui Min Jin

Abstract

Endoplasmic reticulum (ER) stress plays a critical role in the development of diabetic nephropathy (DN). We previously demonstrated that pyruvate (Pyr)-enriched oral rehydration solution improved glucometabolic disorders and ameliorated DN outcome in db/db mice. Here, we investigated the effects of Pyr on high glucose-induced ER stress and apoptosis in HK-2 cells. Our results suggest that high glucose can induce reactive oxygen species production, apoptosis and ER stress in HK-2 cells, and that Pyr treatment can ameliorate these effects and restore the expression of key proteins involved in ER stress. Thus, Pyr may have potential for the development of novel strategies for the prevention and treatment of clinical DN.

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Citations

Mar 12, 2021·Endocrine Reviews·Amir AjoolabadyJun Ren
May 8, 2021·Frontiers in Medicine·Xiaoyun HeChunlin Ou
Aug 17, 2021·Oxidative Medicine and Cellular Longevity·Lihua NiXiaoyan Wu

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Methods Mentioned

BETA
flow cytometry

Software Mentioned

imagej
spss

Related Concepts

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis