PMID: 9546164Apr 18, 1998Paper

Pyruvate decarboxylase catalyzes decarboxylation of branched-chain 2-oxo acids but is not essential for fusel alcohol production by Saccharomyces cerevisiae

Applied and Environmental Microbiology
Eelko G Ter SchureC Theo Verrips

Abstract

The fusel alcohols 3-methyl-1-butanol, 2-methyl-1-butanol, and 2-methyl-propanol are important flavor compounds in yeast-derived food products and beverages. The formation of these compounds from branched-chain amino acids is generally assumed to occur via the Ehrlich pathway, which involves the concerted action of a branched-chain transaminase, a decarboxylase, and an alcohol dehydrogenase. Partially purified preparations of pyruvate decarboxylase (EC 4.1.1.1) have been reported to catalyze the decarboxylation of the branched-chain 2-oxo acids formed upon transamination of leucine, isoleucine, and valine. Indeed, in a coupled enzymatic assay with horse liver alcohol dehydrogenase, cell extracts of a wild-type Saccharomyces cerevisiae strain exhibited significant decarboxylation rates with these branched-chain 2-oxo acids. Decarboxylation of branched-chain 2-oxo acids was not detectable in cell extracts of an isogenic strain in which all three PDC genes had been disrupted. Experiments with cell extracts from S. cerevisiae mutants expressing a single PDC gene demonstrated that both PDC1- and PDC5-encoded isoenzymes can decarboxylate branched-chain 2-oxo acids. To investigate whether pyruvate decarboxylase is essential for fusel ...Continue Reading

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Citations

Sep 25, 1998·The Journal of Biological Chemistry·J R DickinsonM J Hewlins
Jul 26, 2005·Applied Microbiology and Biotechnology·Sung A Schoondermark-StolkLubbert Dijkhuizen
Feb 19, 2008·Applied and Environmental Microbiology·Lucie A HazelwoodJ Richard Dickinson
Nov 19, 2008·Applied Microbiology and Biotechnology·Bart A SmitGerrit Smit
Nov 18, 2014·Frontiers in Microbiology·Joanita SulaimanKok-Gan Chan
Nov 2, 2014·Applied Microbiology and Biotechnology·Waleed Ahmad KhattakJoong Kon Park
Oct 30, 2001·FEMS Microbiology Letters·F AmáritaC Pelaez
Aug 7, 2003·Applied and Environmental Microbiology·Zeynep VuralhanJack T Pronk
Jul 26, 2011·Journal of Industrial Microbiology & Biotechnology·Gustav StygerFlorian F Bauer
Aug 16, 2003·European Journal of Biochemistry·Michel FlipphiBéatrice Felenbok
Jan 7, 2021·Applied Microbiology and Biotechnology·Pwj Labuschagne, B Divol
Mar 30, 2017·International Journal of Molecular Sciences·Jaime Moreno-GarcíaTeresa García-Martínez
May 28, 2004·International Journal of Food Microbiology·Pilar Fernández de PalenciaCarmen Peláez
Apr 25, 2007·Forensic Science International : Synergy·Vassiliki A BoumbaTheodore Vougiouklakis

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