PMID: 7030740Oct 1, 1981Paper

Pyruvate dehydrogenase complex from baker's yeast. 1. Purification and some kinetic and regulatory properties

European Journal of Biochemistry
G B Kresze, H Ronft

Abstract

Pyruvate dehydrogenase complex, for the first time, was highly purified from commercial baker's yeast (saccharomyces cerevisiae). Proteolytic degradation was prevented by the inclusion of the protease inhibitors pepstatin A, leupeptin, and phenylmethanesulfonyl fluoride during the enzyme purification. The yield from 1 kg of pressed yeast was about 15--20 mg enzyme with a specific activity of 17--30 U/mg. Most of the kinetic and regulatory properties of the yeast enzyme were found similar to those of the mammalian mitochondrial pyruvate dehydrogenase complexes except that Km for pyruvate, when assayed at the pH optimum, was much higher than in the mammalian complexes and resembled the values reported for the complexes of gram-negative bacteria. Furthermore, neither in yeast homogenates nor in the isolated yeast pyruvate dehydrogenase complex, was any evidence found for regulation by interconversion (phosphorylation-dephosphorylation) as occurs in mammals, plants, and Neurospora crassa.

References

Nov 15, 1975·European Journal of Biochemistry·T W BrestersC Veeger
Nov 9, 1979·Biochimica Et Biophysica Acta·R KomunieckiH J Saz
Jan 1, 1977·Biochemical Society Transactions·D E GriffithsR L Hyams
Sep 29, 1978·Biochemical and Biophysical Research Communications·C GebhardtH Bisswanger
Sep 15, 1979·Analytical Biochemistry·G B Kresze
Feb 25, 1977·International Archives of Occupational and Environmental Health·D V Roberts
Apr 1, 1975·Journal of Biochemistry·Y HayashiT Totsuka
Nov 15, 1975·European Journal of Biochemistry·T W BrestersC Veeger
Mar 1, 1969·Hoppe-Seyler's Zeitschrift für physiologische Chemie·O WielandB Stukowski
Jun 1, 1970·Archives of Biochemistry and Biophysics·R W HardingR P Wagner
Mar 15, 1972·European Journal of Biochemistry·E A Siess, O H Wieland
May 1, 1972·Journal of Biochemistry·T Hirabayashi, T Harada
Oct 1, 1973·Hoppe-Seyler's Zeitschrift für physiologische Chemie·U WaisJ Ullrich
May 10, 1968·Biochemical and Biophysical Research Communications·E R SchwartzL J Reed
Jan 1, 1969·Biochimica Et Biophysica Acta·M ShimizuY Abiko
Jul 1, 1980·Hoppe-Seyler's Zeitschrift für physiologische Chemie·F Machicao, O H Wieland
May 1, 1980·Journal of Biochemistry·K FujishiroN Katunuma
Jul 31, 1980·Biochemical and Biophysical Research Communications·H Bisswanger
May 1, 1958·Biochimica Et Biophysica Acta·A ALVAREZJ M WIAME
Jan 1, 1961·Cold Spring Harbor Symposia on Quantitative Biology·H HOLZER

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Citations

Aug 20, 1997·Biotechnology and Bioengineering·M RizziM Reuss
Jun 1, 1983·Molecular and Biochemical Parasitology·R KomunieckiM Coulson
Jul 4, 2001·Journal of Biotechnology·F LeiS B Jørgensen
Jul 4, 2001·Journal of Biotechnology·F Lei, S B Jørgensen
Apr 20, 2002·The Biochemical Journal·Tobias ModigMohammad J Taherzadeh
May 21, 2003·Proceedings of the National Academy of Sciences of the United States of America·Yingqi GuJames K Stoops
Jul 3, 2009·The Journal of Biological Chemistry·Melissa S SchonauerCarol L Dieckmann
Nov 6, 2009·Molecular Biology of the Cell·Viktor M BoerJoshua D Rabinowitz
Mar 30, 1990·European Journal of Biochemistry·S Hohmann, H Cederberg
Jan 1, 1989·Annals of the New York Academy of Sciences·L J ReedD J Uhlinger
Aug 24, 2012·Journal of Bioinformatics and Computational Biology·Rajat K De, Namrata Tomar
Jul 30, 2016·Metabolic Engineering·Jifeng Yuan, Chi-Bun Ching
Aug 18, 2009·Biochimica Et Biophysica Acta·R Diaz-RuizM Rigoulet
Jan 1, 1983·Hoppe-Seyler's Zeitschrift für physiologische Chemie·P HeinrichG B Kresze
Aug 15, 1991·Biochemical and Biophysical Research Communications·L P da SilvaH Baltscheffsky
Mar 1, 1991·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·S P FusseyS J Yeaman
Aug 2, 2007·Molecular Systems Biology·Gavin C Conant, Kenneth H Wolfe
Jul 20, 1999·The Journal of Biological Chemistry·S BoubekeurB Guérin
Apr 1, 1985·Journal of Environmental Science and Health. Part. B, Pesticides, Food Contaminants, and Agricultural Wastes·C Madhosingh, W Orr
Apr 12, 2014·Molecular BioSystems·Yifat CohenMaya Schuldiner
Dec 1, 1996·Yeast·J T PronkJ P Van Dijken
Mar 7, 2020·Bulletin of Mathematical Biology·Mohit P Dalwadi, John R King
Feb 1, 1989·Applied and Environmental Microbiology·E PostmaJ P Van Dijken

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