PMID: 7030741Oct 1, 1981Paper

Pyruvate dehydrogenase complex from baker's yeast. 2. Molecular structure, dissociation, and implications for the origin of mitochondria

European Journal of Biochemistry
G B Kresze, H Ronft

Abstract

1. Pyruvate dehydrogenase complex from Saccharomyces cerevisiae is similar in size (s20,w 77 S) and flavin content (1.3--1.4 nmol/mg) to the complexes from mammalian mitochondria. 2. The relative molecular masses of the constituent polypeptide chains, as determined by dodecylsulfate gel electrophoresis at different gel concentrations, were: lipoate acetyltransferase (E2), 58 000; lipoamide dehydrogenase (E3), 56 000; pyruvate dehydrogenase (E1), alpha-subunit, 45 000, and beta-subunit, 35 000. Gel chromatography in the presence of 6 M guanidine . HCl gave a value of 52 000 for E2 indicating anomalous electrophoretic migration as described for the E2 components of other pyruvate dehydrogenase complexes. Thus, the organization and subunit Mr values are similar with the mammalian complexes and virtually identical with the complexes of gram-positive bacteria but differ greatly from the pyruvate dehydrogenase complexes of gram-negative bacteria. 3. The complex was resolved into its component enzymes by the following methods. E1 was obtained by treatment of the complex with elastase followed by gel chromatography on Sepharose CL-2B using a reverse ammonium sulfate gradient for elution. E2 was isolated by gel filtration of the complex...Continue Reading

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Citations

Jan 1, 1983·Molecular & General Genetics : MGG·H D SchmittF K Zimmermann
Feb 21, 2013·Applied Microbiology and Biotechnology·Praveen Kumar AgarwalSantosh B Noronha
Jun 1, 1983·Molecular and Biochemical Parasitology·R KomunieckiM Coulson
Oct 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·X D NiuL J Reed
Mar 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·K S BrowningL J Reed
Apr 16, 1984·European Journal of Biochemistry·H J BosmaC Veeger
Jan 1, 1989·Annals of the New York Academy of Sciences·L J ReedD J Uhlinger
Jan 1, 1983·Hoppe-Seyler's Zeitschrift für physiologische Chemie·P HeinrichG B Kresze
Apr 12, 2014·Molecular BioSystems·Yifat CohenMaya Schuldiner
Dec 1, 1996·Yeast·J T PronkJ P Van Dijken
Mar 7, 2020·Bulletin of Mathematical Biology·Mohit P Dalwadi, John R King
Mar 22, 1990·Journal of Theoretical Biology·K R AlbeB E Wright

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