Pyruvate kinase isoenzyme transitions in cultures of fetal rat hepatocytes

Cell Differentiation and Development : the Official Journal of the International Society of Developmental Biologists
R ScottG C Yeoh


Changes in the expression of two isoenzymic forms of pyruvate kinase in fetal hepatocyte cultures derived from 15- and 19-day gestation rats are studied by immunocytochemical localization of the respective antigens. Initially, in cultures established from 15-day gestation rats only the 'embryonic' form of the enzyme (M2-PK) is detected in all cells. Cells which stain positively for the liver specific form of the enzyme (L-PK) are not observed. After 2 days' culture, a significant number of cells have become positive for L-PK. All the positive cells have a morphology which is typical of liver parenchymal cells. However, the majority of parenchymal cells remain negative for L-PK while retaining M2-PK. In contrast, all cells which display a fibroblastic morphology, as well as clear epithelial cells are M2-PK positive, but L-PK negative. In 5-day-old cultures, all hepatocytes have become L-PK positive. Hepatocytes derived from 19-day gestation rat liver stain positively for L-PK on day 1 of culture in agreement with previously published biochemical data. A minor population of negative cells is non-parenchymal in appearance. All parenchymal cells are negative when the culture is stained with M2-PK specific antibody. Five days after ...Continue Reading


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Jun 1, 1996·The International Journal of Biochemistry & Cell Biology·C M HilliardG C Yeoh
Mar 1, 1994·European Journal of Biochemistry·Z WangT Noguchi
Jul 20, 2004·World Journal of Gastroenterology : WJG·Hai HongGuo-Qiang Zhao

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