Pyruvate kinase isozyme type M2 (PKM2) interacts and cooperates with Oct-4 in regulating transcription

The International Journal of Biochemistry & Cell Biology
Jungwoon LeeJungho Kim

Abstract

The Oct-4 gene encodes a transcription factor that plays an important role in maintaining the pluripotent state of embryonic stem cells and may prevent expression of genes activated during differentiation. Although its role in maintaining embryonic stem cell pluripotency is well established, there is still little known about the binding partners that regulate its function. To identify proteins that control Oct-4 function, we used affinity chromatography on immobilized Oct-4 (POU) together with MALDI-TOF (matrix-assisted laser-desorption ionization-time-of-flight) MS (mass spectrometry) and isolated a novel Oct-4-interacting protein, pyruvate kinase type M2 (PKM2 or M2-PK). PKM2 is an isozyme of pyruvate kinase that is specifically expressed in proliferating cells, such as embryonic stem cells, embryonic carcinoma cells, as well as cancer cells. Oct-4 and PKM2 were co-affinity precipitated from cell extracts, and glutathione S-transferase pull-down assays revealed that the POU DNA binding domain of Oct-4 was required for interaction with PKM2. In addition, the C-terminal domain of PKM2 (amino acids 307-531) was involved in binding to Oct-4. Moreover, ectopic expression of the PKM2 enhanced Oct-4-mediated transcription. These obs...Continue Reading

References

Jan 1, 1975·British Journal of Haematology·S MiwaE Oda
May 1, 1992·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·M DomingoM Reinacher
Mar 29, 1990·Nature·H R SchölerP Gruss
Nov 1, 1991·Mechanisms of Development·Y I YeomK Artzt
Jan 1, 1991·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·P A Oude WeerninkG E Staal
Mar 2, 1989·Nature·J W Lillie, M R Green
Jan 15, 1986·European Journal of Biochemistry·H InoueT Tanaka
Oct 8, 1965·Biochemical and Biophysical Research Communications·T TanakaR Mori
Jan 1, 1982·Acta neurochirurgica·C W van VeelenG E Staal
Jan 1, 1982·Methods in Enzymology·K Imamura, T Tanaka
Mar 28, 1995·Biochemical and Biophysical Research Communications·R UenakaT Tanaka
Jan 1, 1994·Virchows Archiv : an International Journal of Pathology·U BrinckG Fischer
Jan 25, 1993·Nucleic Acids Research·N SuzukiH R Schöler
Aug 6, 1998·Journal of Cancer Research and Clinical Oncology·S KressM Schwarz
Dec 3, 1998·Molecular Human Reproduction·C E Ovitt, H R Schöler
Feb 17, 1999·Proceedings of the National Academy of Sciences of the United States of America·W ZwerschkeP Jansen-Dürr
Mar 17, 1999·International Journal of Cancer. Journal International Du Cancer·T JinP E Goss
Dec 10, 1999·Proceedings of the National Academy of Sciences of the United States of America·J KimJ Pelletier
Oct 25, 2000·Molecular Human Reproduction·C HansisL C Krey

❮ Previous
Next ❯

Citations

Sep 25, 2010·Journal of Neural Transmission·H C BauerH Bauer
Jul 25, 2013·Pathology, Research and Practice·Xuelin ZhangHaihui Sheng
Dec 10, 2013·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Kakali MukherjeeBidyut Saha
Jan 29, 2014·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Rumpa SahaBidyut Saha
Dec 21, 2011·Cell Research·Isaac HarrisTak Wah Mak
Mar 23, 2010·Molecular & Cellular Proteomics : MCP·Anna PrudovaChristopher M Overall
Jan 25, 2012·The Journal of Experimental Medicine·Michael S Goldberg, Phillip A Sharp
Feb 15, 2012·The Journal of Experimental Medicine·Robert B Hamanaka, Navdeep S Chandel
Mar 9, 2013·Nucleic Acids Research·Edward SendlerStephen A Krawetz
Dec 7, 2011·Science·Robert B Hamanaka, Navdeep S Chandel
Mar 13, 2010·Journal of Biomedicine & Biotechnology·H BauerH C Bauer
Sep 4, 2013·The Journal of Clinical Investigation·Gregg L Semenza
Nov 7, 2009·PLoS Computational Biology·Nathan SalomonisBruce R Conklin
Apr 21, 2012·Future Oncology·Frédéric Canal, Christine Perret
Feb 1, 2014·Cell Death & Disease·M MorfouaceF M Vallette
Jan 7, 2014·Molecular Neurobiology·Saima ZafarInga Zerr
Dec 18, 2013·Proceedings of the National Academy of Sciences of the United States of America·Hung-Jung WangWen-Ching Wang
Jul 22, 2011·Stem Cells International·Clara Y Cheong, Thomas Lufkin
Aug 1, 2014·Future Medicinal Chemistry·Steven L WarnerDavid J Bearss
Jun 13, 2012·Cold Spring Harbor Perspectives in Biology·Patrick S Ward, Craig B Thompson
Jun 28, 2014·International Journal of Molecular Sciences·Ling ChenYongguang Tao
Apr 22, 2014·FEBS Letters·Mohd Askandar IqbalRameshwar N K Bamezai
Jul 25, 2012·Trends in Endocrinology and Metabolism : TEM·Weibo Luo, Gregg L Semenza
May 26, 2012·Trends in Biochemical Sciences·Barbara Chaneton, Eyal Gottlieb
Jan 8, 2016·Molecular Cancer·Ninel AzoiteiThomas Seufferlein
Feb 17, 2010·The International Journal of Biochemistry & Cell Biology·Sybille Mazurek
Dec 8, 2015·Scientific Reports·David R ChristensenFranchesca D Houghton
Mar 25, 2009·Journal of Cellular Biochemistry·Gilles A SpodenWerner Zwerschke
Sep 22, 2010·Protein Science : a Publication of the Protein Society·Vibhor Gupta, Rameshwar N K Bamezai
Jun 1, 2010·Proteomics·Rebekah L GundryJennifer E Van Eyk
Aug 15, 2012·The Journal of Obstetrics and Gynaecology Research·Yincheng TengLaimin Luo
Jan 16, 2013·Diseases of the Esophagus : Official Journal of the International Society for Diseases of the Esophagus·C ZhanQ Wang
Mar 21, 2012·Molecular Reproduction and Development·Rebecca L Krisher, Randall S Prather
Jan 1, 2014·Cancer·Shannon E Elf, Jing Chen
Sep 28, 2015·Biochemical and Biophysical Research Communications·Xiao XieLei Lv
Aug 19, 2015·Seminars in Cell & Developmental Biology·William J Israelsen, Matthew G Vander Heiden
Dec 9, 2009·The Biochemical Journal·Jungwoon LeeJungho Kim
Sep 3, 2014·Mitochondrion·Björn KruspigVladimir Gogvadze

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cancer Metabolism

In order for cancer cells to maintain rapid, uncontrolled cell proliferation, they must acquire a source of energy. Cancer cells acquire metabolic energy from their surrounding environment and utilize the host cell nutrients to do so. Here is the latest research on cancer metabolism.