Pyruvate utilization by mouse oocytes is influenced by meiotic status and the cumulus oophorus

Molecular Reproduction and Development
S M DownsH J Leese

Abstract

In this study, the effects of meiotic status on the energy substrate dynamics of mouse oocyte-cumulus cell complexes (OCCs) and denuded oocytes (DOs) have been examined. In the first series of experiments, OCCs from PMSG-primed, immature mice were cultured in minimum essential medium in 8-microl microdrops under a variety of conditions, and the medium and oocytes were sampled for pyruvate and glucose concentration and for meiotic status. Oocytes in control medium underwent germinal vesicle breakdown within 3 hr and the OCCs displayed a time-dependent increase in pyruvate consumption, but the glucose concentration changed very little. Treatment with IBMX or dbcAMP, which maintained complete meiotic arrest, suppressed pyruvate consumption, but slightly more glucose was consumed than in controls. Hypoxanthine (HX) allowed up to 10% of the oocytes to resume maturation, and pyruvate and glucose consumption resembled that of control OCCs. FSH added to HX-containing medium stimulated significant glucose consumption and pyruvate production. In general, a reciprocal relationship was observed between glucose and pyruvate consumption. When the energy substrate dynamics were compared with meiotic status of the oocytes, pyruvate consumption...Continue Reading

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Citations

Aug 2, 2005·Theriogenology·Sarah E HarrisNicolas M Orsi
Nov 11, 2005·Biology of Reproduction·Cean LaRosa, Stephen M Downs
Aug 21, 2013·Reproduction : the Official Journal of the Society for the Study of Fertility·Tessa Lord, R John Aitken
Sep 13, 2008·Reproduction : the Official Journal of the Society for the Study of Fertility·Rachel J WebbAnthony E Michael
Aug 22, 2007·Reproduction : the Official Journal of the Society for the Study of Fertility·Sarah E HarrisHelen M Picton
Sep 27, 2005·Reproduction : the Official Journal of the Society for the Study of Fertility·Kimberly A PreisDavid K Gardner
May 26, 2009·Reproduction : the Official Journal of the Society for the Study of Fertility·Na LiuJing-He Tan
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Jul 12, 2008·Molecular Reproduction and Development·Sarah E HarrisHelen M Picton
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Oct 26, 2010·Molecular and Cellular Endocrinology·Rebecca S BroganCharles L Chaffin
Oct 7, 2006·Developmental Biology·You-Qiang SuJohn J Eppig
Jul 19, 2013·Biology of Reproduction·Deepa Valsangkar, Stephen M Downs
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Dec 20, 2005·Molecular Reproduction and Development·Stephen M DownsHenry J Leese
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