PMID: 3383845Jun 1, 1988Paper

Pyruvic-acid-containing polysaccharide in the cell wall of Bacillus polymyxa AHU 1385

European Journal of Biochemistry
N KojimaE Ito

Abstract

Three acidic polymer fractions with molecular masses of about 16 kDa, 35 kDa and 70 kDa were isolated from lysozyme digests of N-acetylated cell walls of Bacillus polymyxa AHU 1385 by ion-exchange chromatography and gel chromatography. These fractions, containing mannosamine, glucosamine and pyruvic acid in a molar ratio of about 1:1:1 together with glycopeptide components, were characterized as polysaccharide-linked glycopeptides with one, two and more polysaccharide chains. On the other hand, treatment of the cell walls with glycine/HC1 buffer, pH 2.5, at 100 degrees C for 10 min followed by separation of water-soluble products on ion-exchange chromatography gave three polysaccharide fractions, PS-I-III, which contained different amounts of pyruvic acid (0,0.6 and 0.9 residue/mannosamine residue) along with equimolar amounts of mannosamine and glucosamine. Pyruvate-free polysaccharides similar to PS-I were also obtained from PS-II, PS-III and polysaccharide-linked glycopeptides by treatment with 10 mM HC1 at 100 degrees C for 1 h. Results of analyses of these polysaccharide preparations by 1H-NMR and 13C-NMR measurement and methylation, together with data from characterization of fragments obtained by hydrogen fluoride hydrol...Continue Reading

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Citations

Jan 2, 1989·European Journal of Biochemistry·H IwasakiE Ito
Oct 9, 2019·International Journal of Molecular Sciences·Fiona F HagerChristina Schäffer
Jul 28, 2006·The Journal of Biological Chemistry·Biswa ChoudhuryRussell W Carlson

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