Pythium insidiosum keratitis: clinical profile and role of DNA sequencing and zoospore formation in diagnosis

Cornea
Savitri SharmaSisinthy Shivaji

Abstract

To report the molecular and microbiological diagnosis and clinical profile of 13 patients with Pythium insidiosum keratitis. Phase 1 of the study consisted of DNA sequencing of the ITS region of the rDNA of 162 stocked morphologically unidentified nonconsecutive fungal isolates from corneal scraping of patients with keratitis (2010-2012). Blast and phylogenetic analyses of the sequences showed 9 to be P. insidiosum. A retrospective review of archived photographs of colony and direct microscopy of corneal scrapings and clinical records of the cases were performed. Phase 2 began in 2014, in which a simple method of zoospore formation was used for fungal colonies resembling those of P. insidiosum followed by DNA sequencing. The prevalence of P. insidiosum among unidentified fungal isolates from keratitis was 9/162 (5.5%) in phase 1. In phase 2, 4/102 cases (3.9%) of fungal keratitis were identified as P. insidiosum (January-February, 2014). Phylogenetic analysis of all 13 fungal isolates confirmed the identification of P. insidiosum. Corneal infiltrates exhibited hyphate edges, tentacle-like extensions, and dot-like infiltrates surrounding the main infiltrate. Response to topical 5% natamycin eye drops with or without oral antifun...Continue Reading

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Citations

Feb 17, 2017·Cornea·Muralidhar RamappaSunita Chaurasia
Nov 3, 2017·Cornea·Samrat Chatterjee, Deepshikha Agrawal
Nov 28, 2017·Cornea·Orapin AnutarapongpanOlan Suwan-Apichon
Jan 11, 2018·Cornea·Anubha RathiNamrata Sharma
Jun 1, 2018·Journal of Clinical Microbiology·Navaporn WorasilchaiAriya Chindamporn
Mar 17, 2018·The British Journal of Ophthalmology·Bhupesh BaggaPrashant Garg
Sep 15, 2017·The British Journal of Ophthalmology·Shweta AgarwalLily K Therese
Apr 20, 2016·Current Opinion in Ophthalmology·Prashant GargSanhita Roy
Dec 21, 2018·BMJ Case Reports·Anita RaghavanRam Rammohan
May 11, 2019·Medical Mycology·Selva Pandiyan AppavuSiva Ganesa Karthikeyan Rajapandian
Mar 3, 2020·PeerJ·Maria Nina ChitasombatTheerapong Krajaejun
Dec 25, 2019·Current Eye Research·Pranita SahayJeewan S Titiyal
Dec 18, 2019·Mycopathologia·Nitipong PermpalungAriya Chindamporn
Oct 14, 2020·Research in Veterinary Science·Josephine S Y HublinMonica Santin
Oct 19, 2019·Microbial Pathogenesis·Lingzhi NiuYajuan Zheng
Nov 7, 2020·Experimental Eye Research·Lalit Kishore AhirwarBhupesh Bagga
May 2, 2021·Clinical & Experimental Ophthalmology·Himansu S BeheraSavitri Sharma
Apr 30, 2021·Indian Journal of Ophthalmology·Bharat GurnaniJoseph Gubert
Aug 20, 2021·Ocular Immunology and Inflammation·Anahita KateSavitri Sharma
Jun 9, 2020·Ophthalmology·Bhupesh BaggaSanchita Mitra

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