PMID: 9446693Apr 4, 1998Paper

Q-type Ca2+ channels are located closer to secretory sites than L-type channels: functional evidence in chromaffin cells

Pflügers Archiv : European journal of physiology
B LaraA G García

Abstract

This study uses a new strategy to investigate the hypothesis that, of the various Ca2+ channels expressed by a neurosecretory cell, a given channel subtype is coupled more tightly to the exocytotic apparatus than others. The approach is based on the prediction that the degree of inhibition of the secretory response by various Ca2+ channel blockers will differ at low (0.5 mM) and high (5 mM) extracellular Ca2+ concentrations ([Ca2+]o). So, at low [Ca2+]o the K+-evoked catecholamine release from superfused bovine chromaffin cells was depressed 60-70% by 2 microM omega-agatoxin IVA (P/Q-type Ca2+ channel blockade), by 3 microM omega-conotoxin MVIIC (N/P/Q-type Ca2+ channel blockade), or by 3 microM lubeluzole (N/P/Q-type Ca2+ channel blockade); in high [Ca2+]o these blockers inhibited the responses by only 20-35%. At 1-3 microM omega-conotoxin GVIA (N-type Ca2+ channel blockade) or 3 microM furnidipine (L-type Ca2+ channel blockade), secretion was inhibited by 30 and 50%, respectively; such inhibitory effects were similar in low or high [Ca2+]o. Combined furnidipine plus omega-conotoxin MVIIC, omega-agatoxin IVA or omega-conotoxin GVIA exhibited additive blocking effects at both Ca2+ concentrations. The results suggest that Q-type...Continue Reading

Citations

Apr 7, 2009·Pflügers Archiv : European journal of physiology·Juliana M RosaAntonio G García
Apr 2, 2003·Pharmacology & Therapeutics·Philip D Marley
Dec 23, 1999·Current Opinion in Neurobiology·B M OliveraD Yoshikami
Nov 26, 1999·Acta Physiologica Scandinavica·D Aunis, K Langley
Feb 29, 2000·Journal of Neurochemistry·E García-PalomeroA M Cárdenas
Nov 21, 2007·Acta Physiologica·A P FoxZ Xie
May 26, 2007·Proceedings of the National Academy of Sciences of the United States of America·X ZhangL-Y M Huang
May 15, 2012·Journal of Molecular Neuroscience : MN·Juliana M RosaLuis Gandía
Sep 1, 2007·Journal of Neurochemistry·Alvaro O ArdilesAna María Cárdenas
Aug 26, 2016·Pflügers Archiv : European journal of physiology·Ricardo De PascualAntonio G García
Aug 4, 2007·Progress in Neurobiology·Tamás L Török
Sep 4, 2017·Pflügers Archiv : European journal of physiology·Carmen NanclaresLuis Gandía
Nov 20, 2002·Annals of the New York Academy of Sciences·Inmaculada Cuchillo-IbanezAntonio G Garcia
Nov 16, 2010·Journal of Neurochemistry·Yanina D Alvarez, Fernando D Marengo
Nov 3, 2005·The European Journal of Neuroscience·Eva Andrés-MateosCarmen Montiel
Oct 4, 2006·Physiological Reviews·Antonio G GarcíaJavier García-Sancho
Aug 31, 2017·Pflügers Archiv : European journal of physiology·Fernando D Marengo, Ana M Cárdenas
Jul 9, 2017·The Journal of General Physiology·Tejeshwar C RaoArun Anantharam
Sep 16, 2009·The Journal of Physiology·Minnie M WuLeon Lagnado

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