QacR is a repressor protein that regulates expression of the Staphylococcus aureus multidrug efflux pump QacA.

The Journal of Biological Chemistry
S GrkovicR A Skurray

Abstract

The Staphylococcus aureus QacA protein is a multidrug transporter that confers resistance to a broad range of antimicrobial agents via proton motive force-dependent efflux of the compounds. Primer extension analysis was performed to map the transcription start points of the qacA and divergently transcribed qacR mRNAs. Each gene utilized a single promoter element, the locations of which were confirmed by site-directed mutagenesis. Fusions of the qacA and qacR promoters to a chloramphenicol acetyl transferase reporter gene were used to demonstrate that QacR is a trans-acting repressor of qacA transcription that does not autoregulate its own expression. An inverted repeat overlapping the qacA transcription start site was shown to be the operator sequence for control of qacA gene expression. Removal of one half of the operator prevented QacR-mediated repression of the qacA promoter. Purified QacR protein bound specifically to this operator sequence in DNase I-footprinting experiments. Importantly, addition of diverse QacA substrates was shown to induce qacA expression in vivo, as well as inhibit binding of QacR to operator DNA in vitro, by using gel-mobility shift assays. QacR therefore appears to interact directly with structurall...Continue Reading

References

Dec 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·M Lanzer, H Bujard
Dec 4, 1987·Cell·S B Straney, D M Crothers
Feb 1, 1987·Protein Engineering·L J Perry, R Wetzel
Jan 1, 1984·Molecular & General Genetics : MGG·B R LyonR A Skurray
May 1, 1995·Journal of Bacteriology·O LomovskayaA Matin
Jan 1, 1994·Annual Review of Microbiology·W Hillen, C Berens
Apr 21, 1993·Journal of the National Cancer Institute·P M Chaudhary, I B Roninson
Jan 1, 1993·Annual Review of Biochemistry·M M Gottesman, I Pastan
Mar 1, 1994·Trends in Biochemical Sciences·K Lewis
Sep 1, 1995·Antimicrobial Agents and Chemotherapy·X Z LiK Poole
Apr 19, 1996·The Journal of Biological Chemistry·W ShiB P Rosen
Apr 16, 1996·Proceedings of the National Academy of Sciences of the United States of America·I T PaulsenR A Skurray
Jan 1, 1995·Annual Review of Genetics·M M GottesmanI Pastan
Dec 1, 1996·Microbiological Reviews·I T PaulsenR A Skurray
Jan 1, 1997·Clinical and Diagnostic Laboratory Immunology·J L DoranF E Nano
Feb 12, 1998·Protein Science : a Publication of the Protein Society·E E ZheleznovaR G Brennan

❮ Previous
Next ❯

Citations

Oct 24, 2003·Current Opinion in Microbiology·Ian T Paulsen
Jan 22, 2003·Biochemical and Biophysical Research Communications·Lorenzo GalluzziMatti Karp
Jun 9, 1999·Current Biology : CB·K Lewis
May 1, 2009·Journal of Medicinal Chemistry·Michael K GannonMichael R Detty
Aug 16, 2002·Molecular Microbiology·Maria A Schumacher, Richard G Brennan
May 4, 2010·The Journal of Biological Chemistry·Craig DanielsJuan-Luis Ramos
Jun 25, 2003·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Mohammed Mahbub AlamNaoki Watanabe
Feb 28, 2002·The EMBO Journal·Maria A SchumacherRichard G Brennan
Jun 9, 2011·Molecular Plant-microbe Interactions : MPMI·Paola VargasMaría-Trinidad Gallegos
Oct 13, 2006·Current Opinion in Infectious Diseases·H Nikaido, H I Zgurskaya
Jul 1, 2009·Antimicrobial Agents and Chemotherapy·Santiago Ramón-GarcíaJosé A Aínsa
Oct 18, 2006·Antimicrobial Agents and Chemotherapy·Silvia BuroniEdda De Rossi
May 20, 2009·Antimicrobial Agents and Chemotherapy·G E LouwT C Victor
Feb 19, 2000·Antimicrobial Agents and Chemotherapy·A BroounK Lewis
Aug 22, 2000·Antimicrobial Agents and Chemotherapy·N MasudaT Nishino
Sep 25, 2003·Antimicrobial Agents and Chemotherapy·Wilson TeránMaría-Trinidad Gallegos
Feb 25, 2005·Antimicrobial Agents and Chemotherapy·Jun LinQijing Zhang
Apr 28, 2005·Antimicrobial Agents and Chemotherapy·Rungtip ChuanchuenHerbert P Schweizer
Jun 9, 2009·Applied and Environmental Microbiology·Liping WangQijing Zhang
Oct 31, 2000·Applied and Environmental Microbiology·L MereghettiA Audurier
Apr 15, 2003·Clinical Microbiology Reviews·Peter Gilbert, Andrew J McBain
Sep 9, 2008·Journal of Bacteriology·Francisca A Cerda-MairaRonald K Taylor
Nov 13, 2007·Journal of Bacteriology·Herfita AgustiandariArnold J M Driessen
Feb 10, 2009·Journal of Bacteriology·Fernando A MartinAngeles Zorreguieta
Nov 13, 2012·Journal of Bacteriology·Francisca A Cerda-MairaRonald K Taylor
Oct 4, 2011·Journal of Bacteriology·Partho ChattorajIndranil Biswas
May 6, 2003·Journal of Bacteriology·Que Chi Truong-BolducDavid C Hooper
Jul 5, 2005·Journal of Bacteriology·Katherine M HoffmannRichard G Brennan
Sep 7, 2007·Microbiology and Molecular Biology Reviews : MMBR·Jacek LubelskiArnold J M Driessen
Dec 6, 2000·Microbiology and Molecular Biology Reviews : MMBR·M PutmanW N Konings
Nov 29, 2002·Microbiology and Molecular Biology Reviews : MMBR·Steve GrkovicRonald A Skurray
Jun 10, 2005·Microbiology and Molecular Biology Reviews : MMBR·Juan L RamosRaquel Tobes
Sep 28, 2007·Canadian Journal of Microbiology·Adnan A AlatoomJane A Colmer-Hamood
Jun 26, 2007·Journal of Molecular Microbiology and Biotechnology·Karl A HassanMelissa H Brown
Mar 10, 2009·Bioscience, Biotechnology, and Biochemistry·Parichat SukdaMasao Fukuda
Jan 14, 2004·Drugs·Xian-Zhi Li, Hiroshi Nikaido

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.

Bacterial Transport Proteins

Bacterial transport proteins facilitate active and passive transport of small molecules and solutes across the bacterial membrane. Here is the latest research.

Bacterial Transport Proteins (ASM)

Bacterial transport proteins facilitate active and passive transport of small molecules and solutes across the bacterial membrane. Here is the latest research.