QCM-D and ToF-SIMS Investigation to Deconvolute the Relationship between Lipid Adsorption and Orientation on Lipase Activity

Langmuir : the ACS Journal of Surfaces and Colloids
Paul JoyceClive A Prestidge

Abstract

Quartz crystal microbalance with dissipation (QCM-D) and time-of-flight secondary ion mass spectrometry (ToF-SIMS) were used to provide insights into the relationship between lipid adsorption kinetics and molecular behavior in porous silica particles of varying hydrophobicities on lipase activity. Lipase (an interfacial enzyme that cleaves ester bonds to break down lipids to fatty acids and monoglycerides) activity was controlled by loading triglycerides at different surface coverages in hydrophilic and hydrophobic porous silica particles. The rate of lipid adsorption increased 2-fold for the hydrophobic surface compared to the hydrophilic surface. However, for submonolayer lipid coverage, the hydrophilic surface enhanced lipase activity 4-fold, whereas the hydrophobic surface inhibited lipase activity 16-fold, compared to lipid droplets in water. A difference in lipid orientation for low surface coverage, evidenced by ToF-SIMS, indicated that lipid adsorbs to hydrophilic silica in a conformation promoting hydrolysis. Multilayer coverage on hydrophobic and hydrophilic surfaces was indistinguishable with ToF-SIMS analysis. Increased lipid adsorption for both substrates facilitated digestion kinetics comparable to a conventional ...Continue Reading

References

Jul 1, 1978·The British Journal of Nutrition·J A DecuypereH K Henderickx
Feb 22, 1990·Nature·F K WinklerW Hunziker
Dec 30, 1987·European Journal of Biochemistry·B FournetJ F Vliegenthart
Mar 7, 2002·Biochimica Et Biophysica Acta·Martin WickhamAnnette Fillery-Travis
Dec 24, 2002·Biophysical Journal·David TaresteLuc Lebeau
Oct 29, 2003·Biophysical Journal·Ralf RichterAlain Brisson
Aug 23, 2005·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Alfred FahrMathew L S Leigh
Sep 6, 2006·Langmuir : the ACS Journal of Surfaces and Colloids·P ReisH Watzke
Feb 8, 2007·Current Opinion in Clinical Nutrition and Metabolic Care·Martine Armand
Mar 3, 2007·Nature Reviews. Drug Discovery·Christopher J H PorterWilliam N Charman
Oct 13, 2007·Proteins·Hiroyoshi MatsumuraRaja Noor Zaliha Raja Abd Rahman
Aug 12, 2008·Advances in Colloid and Interface Science·P ReisR Miller
Nov 17, 2009·Journal of the American Society for Mass Spectrometry·Ivan M KempsonClive A Prestidge
Jun 10, 2010·Langmuir : the ACS Journal of Surfaces and Colloids·Ivan M KempsonTimothy J Barnes
Feb 11, 2011·Journal of Biomedical Materials Research. Part a·J E BaioDavid G Castner
Aug 16, 2012·Biointerphases·Daniel J Graham, David G Castner
Nov 28, 2013·Langmuir : the ACS Journal of Surfaces and Colloids·Ivan M KempsonClive A Prestidge
Feb 21, 2014·Langmuir : the ACS Journal of Surfaces and Colloids·Paul JoyceClive A Prestidge

❮ Previous
Next ❯

Citations

Mar 10, 2016·Colloids and Surfaces. B, Biointerfaces·Paul JoyceClive A Prestidge
May 28, 2016·Chemical Communications : Chem Comm·Jusheng LuSongqin Liu
Oct 8, 2016·Langmuir : the ACS Journal of Surfaces and Colloids·Nicholas G WelchPaul J Pigram
Apr 9, 2019·Expert Opinion on Drug Delivery·Sajedehsadat MaghrebiPaul Joyce
May 6, 2019·Biotechnology and Applied Biochemistry·Aristodemo CarpenMauro Marengo
Apr 23, 2020·Pharmaceutics·Heejun ParkMin-Soo Kim

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.