QF-PCR-based prenatal detection of aneuploidy in a southeast Asian population

Prenatal Diagnosis
R QuaifeH L Yeo

Abstract

We have investigated the efficacy of using quantitative fluorescent polymerase chain reaction (QF-PCR) for the prenatal recognition of aneuploidy in chromosomes 13, 18, 21, X and Y. A total of 1115 samples, from mainly southeast Asian patients, were analysed and compared in a blind trial to the results previously obtained cytogenetically. A multiplex PCR involving 15 short tandem repeat (STR) sequences was used. The probability of two or more of these markers being informative was calculated, and this required the multiplex PCR to be modified. The QF-PCR and previous cytogenetic results concurred, except for two products of conception (POC). One of these may be a case of complete uniparental disomy that was not recognized cytogenetically. The other was tetraploid, and as such appeared normal using QF-PCR. A mosaic trisomy 18 was correctly identified. The population sample was of a mainly Chinese, ethnic origin, and the allele frequency, size and heterozygosity appeared more restricted than the population groups analysed hitherto. The QF-PCR methodology is an efficient cost-effective method of screening for major chromosome aneuploidy, and, for certain referral categories, could be used alone. It also appears to be applicable to...Continue Reading

Citations

Jan 15, 2011·Genetics in Medicine : Official Journal of the American College of Medical Genetics·Diane J Allingham-HawkinsKathy Chun
Feb 15, 2011·Seminars in Fetal & Neonatal Medicine·Brigitte H W FaasThe-Hung Bui
Mar 1, 2015·Gene·Srinivasan MuthuswamyShubha R Phadke
Sep 5, 2006·Prenatal Diagnosis·Lucia BrownStephen Brown
Jan 24, 2009·Prenatal Diagnosis·Eun Hae ChoEun Hee Lee
Oct 30, 2004·Human Reproduction Update·Umberto NicoliniThe-Hung Bui
Feb 10, 2007·American Journal of Medical Genetics. Part C, Seminars in Medical Genetics·Lisa G Shaffer, The-Hung Bui

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