Oct 21, 2014

QTL mapping of genome regions controlling temephos resistance in larvae of the mosquito Aedes aegypti

PLoS Neglected Tropical Diseases
G Reyes-SolisWilliam C Black

Abstract

The mosquito Aedes aegypti is the principal vector of dengue and yellow fever flaviviruses. Temephos is an organophosphate insecticide used globally to suppress Ae. aegypti larval populations but resistance has evolved in many locations. Quantitative Trait Loci (QTL) controlling temephos survival in Ae. aegypti larvae were mapped in a pair of F3 advanced intercross lines arising from temephos resistant parents from Solidaridad, México and temephos susceptible parents from Iquitos, Peru. Two sets of 200 F3 larvae were exposed to a discriminating dose of temephos and then dead larvae were collected and preserved for DNA isolation every two hours up to 16 hours. Larvae surviving longer than 16 hours were considered resistant. For QTL mapping, single nucleotide polymorphisms (SNPs) were identified at 23 single copy genes and 26 microsatellite loci of known physical positions in the Ae. aegypti genome. In both reciprocal crosses, Multiple Interval Mapping identified eleven QTL associated with time until death. In the Solidaridad×Iquitos (SLD×Iq) cross twelve were associated with survival but in the reciprocal IqxSLD cross, only six QTL were survival associated. Polymorphisms at acetylcholine esterase (AchE) loci 1 and 2 were not ass...Continue Reading

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References

Mentioned in this Paper

Dengue Fever
Metabolic Process, Cellular
Neuro-Oncological Ventral Antigen 2
Chromosomes, Human, Pair 1
Short Tandem Repeat
Choline
Quantitative Trait Loci
Heat-Shock Proteins 70
Genome
Carbamates

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